青少年正常及精索静脉曲张睾丸中的一氧化氮合酶模式

Nitric oxide synthase patterns in normal and varicocele testis in adolescents.

作者信息

Santoro G, Romeo C, Impellizzeri P, Ientile R, Cutroneo G, Trimarchi F, Pedale S, Turiaco N, Gentile C

机构信息

Department of Biomorphology, University of Messina, Messina, Italy.

出版信息

BJU Int. 2001 Dec;88(9):967-73. doi: 10.1046/j.1464-4096.2001.02446.x.

DOI:10.1046/j.1464-4096.2001.02446.x

PMID:11851622

Abstract

OBJECTIVE

To determine if the testes of normal adolescents can produce nitric oxide (NO), by assessing NO synthase (NOS) activity, and whether this activity changes in adolescents with left idiopathic varicocele.

PATIENTS AND METHODS

After obtaining informed consent, testicular biopsies were obtained from eight adolescents (mean age 16.4 years; controls) who underwent surgery for inguinal hernia or hydrocele, and from 20 adolescents (mean age 16.2 years) operated for left idiopathic varicocele. Inducible and endothelial NOS (iNOS and eNOS) isoforms were investigated in the biopsy specimens by immunohistochemical localization and Western blot analysis using specific fluorescein-conjugated antibodies.

RESULTS

Both normal and pathological samples expressed eNOS at the level of vessels and Leydig cells. The iNOS was expressed in Leydig cells of normal testes and over-expressed in Leydig cells of varicocele testes.

CONCLUSION

Leydig cells of adolescent testes constitutively express iNOS. Under pathological conditions, e.g. varicocele, iNOS is up-regulated and is a possible source of NO overproduction. These results could be useful in explaining the pathogenesis of both testis and sperm dysfunction in varicocele.

摘要

目的

通过评估一氧化氮合酶（NOS）活性，确定正常青少年的睾丸是否能产生一氧化氮（NO），以及这种活性在患有左侧特发性精索静脉曲张的青少年中是否会发生变化。

患者与方法

在获得知情同意后，从8名接受腹股沟疝或鞘膜积液手术的青少年（平均年龄16.4岁；对照组）以及20名接受左侧特发性精索静脉曲张手术的青少年（平均年龄16.2岁）获取睾丸活检样本。通过使用特异性荧光素偶联抗体的免疫组织化学定位和蛋白质印迹分析，在活检标本中研究诱导型和内皮型NOS（iNOS和eNOS）亚型。