[雄性大鼠睾丸中一氧化氮合酶的表达]

[The expression of nitric oxide synthase in testes of male rat].

作者信息

Li Mei-Xiang, He Li-Ping, Guo Zi-Qian, Liu Yue-Shun, Long Zhi-Feng

机构信息

Department of Histology and Embryology, Nanhua University, Hengyang, Hunan 421001, China.

出版信息

Zhonghua Nan Ke Xue. 2002;8(4):250-2.

PMID:12491685

Abstract

OBJECTIVES

To determine the expression of nitric oxide synthase (NOS) in testis and to investigate the effects of NO on the reproductive function of testis.

METHODS

Testes of adult male Sprague-Dawley rats were fixed in 4% paraformaldehyde. The paraffin sections were made as routine. Immunohistochemical ABC method was used to observe the localization of NOS.

RESULTS

Endothelia NOS (eNOS), neuronal NOS (nNOS) and inductive NOS (iNOS) were all expressed in Leydig cells. Only eNOS was expressed in peritubular myoid cells, endothelial and smooth muscle cells of blood vessel, while only nNOS expressed in tunica adventitia of testicular blood vessels. The reactive substance distributes in cytoplasm with negative nuclei. Immunoreactivity for eNOS, nNOS and iNOS in all spermatogenic cells was negative.

CONCLUSIONS

Three kinds of NOS were all expressed in testis and the distribution of different NOS had a little difference.

摘要

目的

测定一氧化氮合酶（NOS）在睾丸中的表达，并探讨一氧化氮（NO）对睾丸生殖功能的影响。

方法

将成年雄性Sprague-Dawley大鼠的睾丸用4%多聚甲醛固定。按常规方法制作石蜡切片。采用免疫组织化学ABC法观察NOS的定位。

结果

内皮型一氧化氮合酶（eNOS）、神经型一氧化氮合酶（nNOS）和诱导型一氧化氮合酶（iNOS）均在睾丸间质细胞中表达。仅eNOS在睾丸肌样细胞、血管内皮和平滑肌细胞中表达，而仅nNOS在睾丸血管外膜中表达。反应物质分布于细胞质中，细胞核呈阴性。所有生精细胞中eNOS、nNOS和iNOS的免疫反应均为阴性。