Montes Sergio, Alcaraz-Zubeldia Mireya, Ríos Camilo, Muriel Pablo
Departamento de Neuroquímica, Instituto Nacional de Neurología y Neurocirugía, Manuel Velasco Suarez, D.F., México, Mexico.
Brain Res Brain Res Protoc. 2002 Feb;9(1):9-15. doi: 10.1016/s1385-299x(01)00132-5.
Cirrhotic patients tend to accumulate manganese in their brain, especially in basal ganglia. Manganese is a well-known neurotoxic metal, however, its effect in a condition such as liver damage has not been explored deeply due to the lack of a suitable experimental model. A method to induce manganese accumulation in the brain of the cirrhotic rat is described. Cirrhosis was induced by obstruction of biliary duct and simultaneous treatment with manganese in the drinking water (0.5 or 1 mg/ml) during 4 weeks. Metal brain accumulation was low in sham-operated rats with both of the Mn concentrations used. In contrast, manganese treatment to bile obstructed rats resulted in fourfold and eightfold metal increments in the 0.5 and 1 mg/ml Mn2+ concentrations, respectively. This method is useful to induce brain manganese deposition and to study its consequences.
肝硬化患者的大脑往往会蓄积锰，尤其是在基底神经节。锰是一种众所周知的神经毒性金属，然而，由于缺乏合适的实验模型，其在肝损伤等情况下的作用尚未得到深入研究。本文描述了一种在肝硬化大鼠大脑中诱导锰蓄积的方法。通过胆管阻塞诱导肝硬化，并在4周内同时用饮用水中的锰（0.5或1毫克/毫升）进行处理。使用的两种锰浓度在假手术大鼠中大脑金属蓄积量都很低。相比之下，对胆管阻塞大鼠进行锰处理后，在0.5毫克/毫升和1毫克/毫升的锰离子浓度下，金属含量分别增加了四倍和八倍。该方法有助于诱导大脑锰沉积并研究其后果。
