Smith Christopher P, Somogyi George T, Bird Erin T, Chancellor Michael B, Boone Timothy B
Department of Urology, Baylor College of Medicine and the Veterans Affairs Spinal Cord Injury Unit, Houston TX, USA.
Brain Res Brain Res Protoc. 2002 Feb;9(1):57-64. doi: 10.1016/s1385-299x(01)00137-4.
We describe an animal model to study neurotransmitter changes in parallel with urodynamic testing following Spinal Cord Injury (SCI). Urodynamic access was achieved using a subcutaneously placed 7 French dual lumen portacatheter. Spinal cord injury was induced by weight drop technique onto exposed dura at T8. The L6-S1 detrusor nuclei were localized stereotactically and microdialysis probe placement was confirmed through histologic methods. Chronic urodynamics revealed detrusor hyperreflexia (DH) 14 days following SCI. In vivo microdialysis of spinal cord amino acids was performed using CMA 11 (240 uM) probes in halothane-anesthetized rats at baseline and intervals of 20-30 min following spinal cord injury. Significant increases in the excitatory amino acid glutamate, and the inhibitory amino acids, glycine and taurine, were seen following spinal cord injury. Amino acid levels peaked at approximately 40 min following contusion injury with glycine demonstrating the highest levels of all amino acids measured. This neurogenic rat model provides a useful means of examining the effects of spinal cord injury on bladder function. By utilizing spinal cord microdialysis, one could intervene at the level of the detrusor nuclei to modulate bladder function.
我们描述了一种动物模型，用于在脊髓损伤（SCI）后与尿动力学测试同时研究神经递质变化。通过皮下放置的7 French双腔端口导管实现尿动力学通路。采用重物坠落技术在T8暴露的硬脊膜上诱导脊髓损伤。通过立体定向定位L6 - S1逼尿肌核，并通过组织学方法确认微透析探针的放置。慢性尿动力学显示SCI后14天出现逼尿肌反射亢进（DH）。在基线以及脊髓损伤后20 - 30分钟的间隔时间，使用CMA 11（240 μM）探针在氟烷麻醉的大鼠中对脊髓氨基酸进行体内微透析。脊髓损伤后，兴奋性氨基酸谷氨酸以及抑制性氨基酸甘氨酸和牛磺酸显著增加。挫伤性损伤后约40分钟氨基酸水平达到峰值，其中甘氨酸在所有测量的氨基酸中水平最高。这种神经源性大鼠模型为研究脊髓损伤对膀胱功能的影响提供了一种有用的方法。通过利用脊髓微透析，可以在逼尿肌核水平进行干预以调节膀胱功能。
