García-Cañas Virginia, González Ramón, Cifuentes Alejandro
Institute of Industrial Fermentations (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
J Agric Food Chem. 2002 Feb 27;50(5):1016-21. doi: 10.1021/jf011033g.
In this paper, the possibilities of capillary gel electrophoresis (CGE) to detect transgenic maize in flours are shown. The method is based on the extraction and amplification by the polymerase chain reaction (PCR) of a specific DNA fragment from transgenic maize and its subsequent analysis by CGE with UV detection or laser-induced fluorescence (LIF). Some useful considerations regarding the optimization of DNA extraction and amplification conditions are given. Also, a comparison is established between the two CGE protocols for DNA detection based on ultraviolet absorption (CGE-UV) and LIF (CGE-LIF). The requirements, advantages, and limitations of both CGE methods are discussed. To our knowledge, this is the first paper on the use of CGE-LIF to detect transgenic food.
本文展示了毛细管凝胶电泳（CGE）检测面粉中转基因玉米的可能性。该方法基于通过聚合酶链反应（PCR）从转基因玉米中提取和扩增特定DNA片段，随后用紫外检测或激光诱导荧光（LIF）的CGE对其进行分析。给出了一些关于优化DNA提取和扩增条件的有益考虑。此外，还对基于紫外吸收（CGE-UV）和LIF（CGE-LIF）的两种DNA检测CGE方案进行了比较。讨论了两种CGE方法的要求、优点和局限性。据我们所知，这是第一篇关于使用CGE-LIF检测转基因食品的论文。
