Pan Z, Zhang W, Wu Y
Beijing Institute of Ophthalmology, Beijing 100005, China.
Zhonghua Yan Ke Za Zhi. 2000 Jan;36(1):32-5, 3.
To study the treatment of rabbit limbal alkali burn by cultured limbal stem cells growing on amniotic membrane for allograft transplantation.
After the primary culture of the rabbit corneal stem cells, they were cultured on amniotic membrane in DMEM/HamF12 medium for one week. Corneal stem cells and amniotic membrane were transplanted on the limbal and scleral area of the rabbit model with alkali burn. The corneal changes were observed by a slitlamp everyday, and the corneal pathological changes were examined.
Cultured rabbit corneal stem cells continued to proliferate, differentiate and form multiple cell layers on amniotic membrane. After transplantation with cultured stem cells and amniotic membrane, the rabbit epithelium showed corneal phenotype and progressive decrease of vascularity and stromal infiltration in the limbal and peripheral zone. Pathological examination verified that the limbal and peripheral corneal epithelium was composed of multilayer cells, the neo-vascularization was reduced and stromal inflammatory cells were decreased.
Allograft transplantation with cultured limbal stem cells can restore corneal epithelial cell composition, decrease neovascularization, maintain limbal cellular barrier function and provide better condition for keratoplasty later.
研究培养于羊膜上的角膜缘干细胞异体移植治疗兔角膜缘碱烧伤的效果。
兔角膜干细胞原代培养后，在DMEM/HamF12培养基中于羊膜上培养1周。将角膜干细胞和羊膜移植到兔碱烧伤模型的角膜缘和巩膜区域。每天用裂隙灯观察角膜变化，并检查角膜病理改变。
培养的兔角膜干细胞在羊膜上继续增殖、分化并形成多层细胞。移植培养的干细胞和羊膜后，兔角膜上皮呈现角膜表型，角膜缘和周边区域血管化程度逐渐降低，基质浸润减少。病理检查证实角膜缘和周边角膜上皮由多层细胞组成，新生血管减少，基质炎症细胞减少。
培养的角膜缘干细胞异体移植可恢复角膜上皮细胞组成，减少新生血管形成，维持角膜缘细胞屏障功能，并为后期角膜移植提供更好的条件。
