纤连蛋白结合蛋白对猪链球菌2型发病机制的作用
Contribution of fibronectin-binding protein to pathogenesis of Streptococcus suis serotype 2.
作者信息
de Greeff Astrid, Buys Herma, Verhaar Robin, Dijkstra Janny, van Alphen Loek, Smith Hilde E
机构信息
Department of Medical Microbiology, University of Amsterdam, The Netherlands.
出版信息
Infect Immun. 2002 Mar;70(3):1319-25. doi: 10.1128/IAI.70.3.1319-1325.2002.
In the present study we investigated the role of the fibronectin (FN)- and fibrinogen (FGN)-binding protein (FBPS) in the pathogenesis of Streptococcus suis serotype 2 in piglets. The complete gene encoding FBPS from S. suis serotype 2 was cloned in Escherichia coli and sequenced. The occurrence of the gene in various serotypes was analyzed by hybridization studies. The FBPS protein was expressed in E. coli and purified, and binding to human FN and FGN was demonstrated. The induction of antibodies in piglets was studied upon infection. An isogenic mutant unable to produce FBPS was constructed, and the levels of virulence of the wild-type and mutant strains were compared in a competitive infection model in young piglets. Organ cultures showed that FBPS was not required for colonization of the tonsils but that FBPS played a role in the colonization of the specific organs involved in an S. suis infection. Therefore, the FBPS mutant was considered as an attenuated mutant.
在本研究中，我们调查了纤连蛋白（FN）和纤维蛋白原（FGN）结合蛋白（FBPS）在猪链球菌2型仔猪发病机制中的作用。将来自猪链球菌2型的编码FBPS的完整基因克隆到大肠杆菌中并进行测序。通过杂交研究分析该基因在各种血清型中的出现情况。FBPS蛋白在大肠杆菌中表达并纯化，证实其与人FN和FGN结合。研究了仔猪感染后抗体的诱导情况。构建了一个不能产生FBPS的同基因突变体，并在幼龄仔猪的竞争感染模型中比较了野生型和突变株的毒力水平。器官培养表明，扁桃体定植不需要FBPS，但FBPS在猪链球菌感染所涉及的特定器官的定植中发挥作用。因此，FBPS突变体被认为是减毒突变体。
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