• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

缺乏溶菌酶的猪链球菌特定突变体的产生与特性分析

Generation and characterization of a defined mutant of Streptococcus suis lacking suilysin.

作者信息

Allen A G, Bolitho S, Lindsay H, Khan S, Bryant C, Norton P, Ward P, Leigh J, Morgan J, Riches H, Eastty S, Maskell D

机构信息

Centre for Veterinary Science, Department of Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 0ES, United Kingdom.

出版信息

Infect Immun. 2001 Apr;69(4):2732-5. doi: 10.1128/IAI.69.4.2732-2735.2001.

DOI:10.1128/IAI.69.4.2732-2735.2001
PMID:11254643
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC98215/
Abstract

A defined allelic-replacement mutant of the sly gene, encoding a thiol-activated cytolysin, from a European isolate of Streptococcus suis serotype 2 was generated and characterized. Unlike the parental strain, it is nonhemolytic, noncytotoxic for cultured macrophage-like cells, avirulent in a mouse infection model, yet only slightly attenuated in a porcine model of systemic infection.

摘要

构建并鉴定了一株来自欧洲2型猪链球菌分离株的sly基因（编码一种硫醇激活的溶细胞素）的明确等位基因替换突变体。与亲本菌株不同，它不溶血，对培养的巨噬细胞样细胞无细胞毒性，在小鼠感染模型中无毒力，但在猪全身感染模型中仅略有减毒。

相似文献

1
Generation and characterization of a defined mutant of Streptococcus suis lacking suilysin.缺乏溶菌酶的猪链球菌特定突变体的产生与特性分析
Infect Immun. 2001 Apr;69(4):2732-5. doi: 10.1128/IAI.69.4.2732-2735.2001.
2
Role of suilysin in pathogenesis of Streptococcus suis capsular serotype 2.溶菌酶在猪链球菌2型荚膜血清型发病机制中的作用。
Microb Pathog. 2003 Jan;34(1):27-37. doi: 10.1016/s0882-4010(02)00192-4.
3
Putative mannose-specific phosphotransferase system component IID represses expression of suilysin in serotype 2 Streptococcus suis.假定的甘露糖特异性磷酸转移酶系统组分IID抑制2型猪链球菌中溶菌酶的表达。
Vet Microbiol. 2005 Feb 25;105(3-4):169-80. doi: 10.1016/j.vetmic.2004.10.017. Epub 2004 Dec 24.
4
Prevalence of the suilysin gene in Streptococcus suis strains isolated from diseased and healthy carrier pigs.从患病猪和健康携带猪中分离出的猪链球菌菌株中溶菌酶基因的流行情况。
Pol J Vet Sci. 2005;8(2):141-5.
5
Distribution and genetic diversity of suilysin in Streptococcus suis isolated from different diseases of pigs and characterization of the genetic basis of suilysin absence.从患有不同疾病的猪中分离出的猪链球菌中溶菌酶的分布与遗传多样性以及溶菌酶缺失的遗传基础特征
Infect Immun. 2001 Dec;69(12):7572-82. doi: 10.1128/IAI.69.12.7572-7582.2001.
6
Production of muraminidase-released protein (MRP), extracellular factor (EF) and suilysin by field isolates of Streptococcus suis capsular types 2, 1/2, 9, 7 and 3 isolated from swine in France.从法国猪群中分离出的2型、1/2型、9型、7型和3型猪链球菌田间分离株产生胞壁质酶释放蛋白（MRP）、细胞外因子（EF）和溶菌酶。
Vet Res. 2000 Sep-Oct;31(5):473-9. doi: 10.1051/vetres:2000133.
7
The genetically modified suilysin, rSLY(P353L), provides a candidate vaccine that suppresses proinflammatory response and reduces fatality following infection with Streptococcus suis.基因改造的苏氨酸溶素 rSLY(P353L) 提供了一种候选疫苗，能抑制猪链球菌感染后的炎症反应并降低死亡率。
Vaccine. 2013 Aug 28;31(38):4209-15. doi: 10.1016/j.vaccine.2013.07.004. Epub 2013 Jul 12.
8
Disruption of srtA gene in Streptococcus suis results in decreased interactions with endothelial cells and extracellular matrix proteins.猪链球菌中srtA基因的破坏导致与内皮细胞和细胞外基质蛋白的相互作用减少。
Vet Microbiol. 2008 Mar 18;127(3-4):417-24. doi: 10.1016/j.vetmic.2007.08.032. Epub 2007 Sep 18.
9
Characterisation of the gene encoding suilysin from Streptococcus suis and expression in field strains.猪链球菌溶血素基因的鉴定及其在田间菌株中的表达
FEMS Microbiol Lett. 1998 Oct 15;167(2):255-61. doi: 10.1111/j.1574-6968.1998.tb13236.x.
10
Presence of the Streptococcus suis suilysin gene and expression of MRP and EF correlates with high virulence in Streptococcus suis type 2 isolates.猪链球菌溶素基因的存在以及MRP和EF的表达与2型猪链球菌分离株的高毒力相关。
Vet Microbiol. 1999 Dec;70(3-4):201-11. doi: 10.1016/s0378-1135(99)00147-9.

引用本文的文献

1
Population Genomics, Virulence Traits, and Antimicrobial Resistance of Isolated in China.中国分离株的群体基因组学、毒力特征及抗菌药物耐药性
Microorganisms. 2025 May 23;13(6):1197. doi: 10.3390/microorganisms13061197.
2
Cholesterol dependent cytolysins and the brain: Revealing a potential therapeutic avenue for bacterial meningitis.胆固醇依赖性细胞溶素与大脑：揭示细菌性脑膜炎的潜在治疗途径。
AIMS Microbiol. 2023 Aug 21;9(4):647-667. doi: 10.3934/microbiol.2023033. eCollection 2023.
3
Screening for Virulence-Related Genes via a Transposon Mutant Library of Serotype 2 Using a Larvae Infection Model.利用幼虫感染模型通过2型血清型转座子突变体文库筛选毒力相关基因
Microorganisms. 2022 Apr 21;10(5):868. doi: 10.3390/microorganisms10050868.
4
Identification of putative zoonotic virulence factors: A systematic review and genomic meta-analysis.鉴定可能的人畜共患病毒力因子：系统评价和基因组荟萃分析。
Virulence. 2021 Dec;12(1):2787-2797. doi: 10.1080/21505594.2021.1985760.
5
Effective Antibacterial and Antihemolysin Activities of Ellipticine Hydrochloride against Streptococcus suis in a Mouse Model.盐酸依匹斯汀在小鼠模型中对猪链球菌的有效抗菌和抗溶血活性。
Appl Environ Microbiol. 2021 Apr 27;87(10). doi: 10.1128/AEM.03165-20.
6
Proposed virulence-associated genes of Streptococcus suis isolates from the United States serve as predictors of pathogenicity.来自美国的猪链球菌分离株中拟与毒力相关的基因可作为致病性的预测指标。
Porcine Health Manag. 2021 Feb 28;7(1):22. doi: 10.1186/s40813-021-00201-6.
7
Phenotype and multi-omics comparison of Staphylococcus and Streptococcus uncovers pathogenic traits and predicts zoonotic potential.葡萄球菌属和链球菌属的表型和多组学比较揭示了其致病特征，并预测了其潜在的人畜共患病性。
BMC Genomics. 2021 Feb 4;22(1):102. doi: 10.1186/s12864-021-07388-6.
8
Research: Progress and Challenges.研究：进展与挑战。
Pathogens. 2020 Aug 27;9(9):707. doi: 10.3390/pathogens9090707.
9
Interaction of Macrophages and Cholesterol-Dependent Cytolysins: The Impact on Immune Response and Cellular Survival.巨噬细胞与胆固醇依赖性细胞溶素的相互作用：对免疫反应和细胞存活的影响
Toxins (Basel). 2020 Aug 19;12(9):531. doi: 10.3390/toxins12090531.
10
Update on Research and Prevention in the Era of Antimicrobial Restriction: 4th International Workshop on .抗菌药物限制时代的研究与预防最新进展：第四届国际研讨会
Pathogens. 2020 May 14;9(5):374. doi: 10.3390/pathogens9050374.

本文引用的文献

1
Streptococcus suis serotype 2 interactions with human brain microvascular endothelial cells.猪链球菌2型与人脑微血管内皮细胞的相互作用。
Infect Immun. 2000 Feb;68(2):637-43. doi: 10.1128/IAI.68.2.637-643.2000.
2
Epithelial invasion and cell lysis by virulent strains of Streptococcus suis is enhanced by the presence of suilysin.猪链球菌强毒株的上皮侵袭和细胞裂解因溶菌酶的存在而增强。
FEMS Immunol Med Microbiol. 1999 Oct;26(1):25-35. doi: 10.1111/j.1574-695X.1999.tb01369.x.
3
Heat-killed Streptococcus suis capsular type 2 strains stimulate tumor necrosis factor alpha and interleukin-6 production by murine macrophages.热灭活的2型猪链球菌菌株可刺激小鼠巨噬细胞产生肿瘤坏死因子α和白细胞介素-6。
Infect Immun. 1999 Sep;67(9):4646-54. doi: 10.1128/IAI.67.9.4646-4654.1999.
4
Characterisation of the gene encoding suilysin from Streptococcus suis and expression in field strains.猪链球菌溶血素基因的鉴定及其在田间菌株中的表达
FEMS Microbiol Lett. 1998 Oct 15;167(2):255-61. doi: 10.1111/j.1574-6968.1998.tb13236.x.
5
Protection of experimentally infected pigs by suilysin, the thiol-activated haemolysin of Streptococcus suis.猪链球菌硫醇激活溶血素——溶菌酶对实验感染猪的保护作用
Vet Rec. 1996 Sep 7;139(10):225-8. doi: 10.1136/vr.139.10.225.
6
Production of suilysin, the thiol-activated haemolysin of Streptococcus suis, by field isolates from diseased pigs.来自患病猪的田间分离株产生猪链球菌的硫醇激活溶血素——溶菌酶。
Vet Rec. 1995 Sep 16;137(12):295-6. doi: 10.1136/vr.137.12.295.
7
Identification, purification, and characterization of a thiol-activated hemolysin (suilysin) of Streptococcus suis.猪链球菌硫醇激活溶血素（猪溶素）的鉴定、纯化及特性分析
Infect Immun. 1994 May;62(5):1742-8. doi: 10.1128/iai.62.5.1742-1748.1994.
8
Partial characterization of Streptococcus suis type 2 hemolysin.猪链球菌2型溶血素的部分特性分析
J Clin Microbiol. 1994 May;32(5):1256-60. doi: 10.1128/jcm.32.5.1256-1260.1994.
9
Characterization of Streptococcus suis capsular type 2 haemolysin.猪链球菌2型溶血素的特性分析
Microbiology (Reading). 1995 Jan;141 ( Pt 1):189-95. doi: 10.1099/00221287-141-1-189.
10
The limited role of pneumolysin in the pathogenesis of pneumococcal meningitis.肺炎溶血素在肺炎球菌性脑膜炎发病机制中的作用有限。
J Infect Dis. 1995 Sep;172(3):805-9. doi: 10.1093/infdis/172.3.805.