替代性σ因子（28）对嗜肺军团菌毒力和鞭毛表达的影响。
Influence of the alternative sigma(28) factor on virulence and flagellum expression of Legionella pneumophila.
作者信息
Heuner Klaus, Dietrich Claudia, Skriwan Carina, Steinert Michael, Hacker Jörg
机构信息
Institut für Molekulare Infektionsbiologie, Universität Würzburg, 97070 Würzburg, Germany.
出版信息
Infect Immun. 2002 Mar;70(3):1604-8. doi: 10.1128/IAI.70.3.1604-1608.2002.
Abstract
The fliA gene of Legionella pneumophila encoding the alternative sigma(28) factor was inactivated by introducing a kanamycin resistance cassette. Electron microscopy and Western blot analysis revealed that the fliA mutant strain is aflagellate and expresses no flagellin. Reporter gene assays indicated that the flaA promoter is not active in the fliA mutant strain. The fliA mutant strain multiplied less effectively in coculture with amoebae than the wild-type strain and was not able to replicate in coculture with Dictyostelium discoideum.
摘要
通过导入卡那霉素抗性盒使嗜肺军团菌编码替代σ（28）因子的fliA基因失活。电子显微镜和蛋白质免疫印迹分析表明，fliA突变株无鞭毛且不表达鞭毛蛋白。报告基因检测表明，flaA启动子在fliA突变株中无活性。与野生型菌株相比，fliA突变株在与变形虫共培养时繁殖效率较低，并且无法在与盘基网柄菌共培养时进行复制。
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