Factors Mediating Environmental Biofilm Formation by .

() is an opportunistic waterborne pathogen and the causative agent for Legionnaires' disease, which is transmitted to humans via inhalation of contaminated water droplets. The bacterium is able to colonize a variety of man-made water systems such as cooling towers, spas, and dental lines and is widely distributed in multiple niches, including several species of protozoa In addition to survival in planktonic phase, is able to survive and persist within multi-species biofilms that cover surfaces within water systems. Biofilm formation by is advantageous for the pathogen as it leads to persistence, spread, resistance to treatments and an increase in virulence of this bacterium. Furthermore, Legionellosis outbreaks have been associated with the presence of in biofilms, even after the extensive chemical and physical treatments. In the microbial consortium-containing among other organisms, several factors either positively or negatively regulate the presence and persistence of in this bacterial community. Biofilm-forming is of a major importance to public health and have impact on the medical and industrial sectors. Indeed, prevention and removal protocols of as well as diagnosis and hospitalization of patients infected with this bacteria cost governments billions of dollars. Therefore, understanding the biological and environmental factors that contribute to persistence and physiological adaptation in biofilms can be detrimental to eradicate and prevent the transmission of . In this review, we focus on various factors that contribute to persistence of within the biofilm consortium, the advantages that the bacteria gain from surviving in biofilms, genes and gene regulation during biofilm formation and finally challenges related to biofilm resistance to biocides and treatments.