Kazama Shinsuke, Ajioka Yoichi, Watanabe Hidenobu, Watanabe Toshiaki, Nagawa Hirokazu
First Department of Pathology, School of Medicine, Niigata University, Niigata City, PO Box 951-8510, Japan.
Jpn J Cancer Res. 2002 Feb;93(2):178-83. doi: 10.1111/j.1349-7006.2002.tb01256.x.
The aim of this study was to elucidate whether K-ras (codons 12 and 13) mutations occur in depressed-type early colorectal carcinomas (DECas) larger than 10 mm in size. Thirty-four cases of DECas including 27 larger than 10 mm were examined for K-ras mutations by means of microdissection, PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism), and direct sequencing. Although K-ras mutation was infrequent (1/7, 14%) in small (less than 10 mm) DECas, 16/27 (59%) and 17/27 (63%) of DECas larger than 10 mm revealed codon 12, or either codon 12 or 13 mutations, respectively. None of the evaluated pathological factors except size showed a correlation with K-ras mutation. These data indicate that although K-ras mutation could not be involved in the early stage of the progression of DECas, it might play a role at a later stage when the tumor size is over 10 mm.
本研究旨在阐明K-ras（密码子12和13）突变是否发生于直径大于10 mm的凹陷型早期结直肠癌（DECas）中。通过显微切割、聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和直接测序，对34例DECas（包括27例直径大于10 mm的病例）进行K-ras突变检测。尽管K-ras突变在小（小于10 mm）的DECas中不常见（1/7，14%），但直径大于10 mm的DECas中分别有16/27（59%）和17/27（63%）显示密码子12或密码子12和13的突变。除大小外，所评估的病理因素均与K-ras突变无相关性。这些数据表明，尽管K-ras突变可能不参与DECas进展的早期阶段，但当肿瘤大小超过10 mm时，它可能在后期发挥作用。
