Wang Yu, Tian Fuzhou, Tang Lijun, Huang Darong, Li Xiaojun, Yin Zhiliang
PLA Center of General Surgery, General Hospital of Chengdu Military Command, Chengdu 610083, China.
Zhonghua Gan Zang Bing Za Zhi. 2002 Feb;10(1):21-4.
To evaluate the effect of glycogen on calcium concentration of rabbit donor liver during ischemia-reperfusion period.
Donor group (n=21) was divided into 3 subgroups randomly: Group A (n=7): fasting for 24 hours before harvesting; Group B (n=7): normal laboratory chew; Group C (n=7): normal laboratory chew plus glucose supplement intravenously. Based on the self-created animal model for ischemia-reperfusion, the levels of glycogen content, ATP level, viability of Ca(2+)ATPase and plasmic free Ca(2+) concentration ([Ca(2+)]i) of liver tissue were measured.
Before cold preservation, there was a significant difference of glycogen content among the three groups at all time points except at the end of rewarming period. ATP level and Ca(2+)ATPase viability were significantly higher in group C than in other two groups. But the plasmic free Ca(2+) concentration was lower in groups with higher glycogen content.
Donor liver with high glycogen content can provide relatively sufficient ATP, maintain better Ca(2+)ATPase viability and prevent plasmic free Ca(2+) concentration overloading. This maybe an important mechanism for glycogen to ameliorate ischemia-reperfusion injury to the donor livers.
评估糖原对兔供肝缺血再灌注期钙浓度的影响。
供体组（n = 21）随机分为3个亚组：A组（n = 7）：取肝前禁食24小时；B组（n = 7）：正常实验室饮食；C组（n = 7）：正常实验室饮食加静脉补充葡萄糖。基于自行建立的缺血再灌注动物模型，检测肝组织糖原含量、ATP水平、Ca(2+)ATP酶活性及血浆游离Ca(2+)浓度（[Ca(2+)]i）。
冷保存前，除复温期末外，各时间点三组糖原含量均有显著差异。C组ATP水平和Ca(2+)ATP酶活性显著高于其他两组。但糖原含量较高组的血浆游离Ca(2+)浓度较低。
糖原含量高的供肝能提供相对充足的ATP，维持较好的Ca(2+)ATP酶活性，防止血浆游离Ca(2+)浓度过载。这可能是糖原减轻供肝缺血再灌注损伤的重要机制。
