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嗜热厌氧糖解热厌氧菌β-木糖苷酶的结晶及初步X射线晶体学分析

Crystallization and preliminary X-ray crystallographic analysis of beta-xylosidase from Thermoanaerobacterium saccharolyticum, a thermophilic anaerobe.

作者信息

Yang Jin Kuk, Yoon Hye-Jin, Ahn Hyung Jun, Lee Byung Il, Kim Hyung-Wook, Laivenieks Maris, Vieille Claire, Zeikus J Gregory, Suh Se Won

机构信息

National Research Laboratory of Structural Proteomics, School of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, South Korea.

出版信息

Acta Crystallogr D Biol Crystallogr. 2002 Mar;58(Pt 3):531-2. doi: 10.1107/s0907444902000227. Epub 2002 Feb 21.

Abstract

beta-Xylosidases are involved in the breakdown of xylans into xylose and belong to either family 39 or 43 of the glycosyl hydrolases. At present, no structural information is available for any member of these families. beta-Xylosidase from the thermophilic anaerobe Thermoanaerobacterium saccharolyticum, a member of glycosyl hydrolase family 39, has been crystallized at 296 K using the hanging-drop vapour-diffusion method. The crystal diffracts to 2.4 A resolution with synchrotron X-rays and belongs to space group P4(1)2(1)2 (or P4(3)2(1)2), with unit-cell parameters a = b = 92.75, c = 241.37 A. The asymmetric unit contains two monomers of the recombinant enzyme, giving a corresponding V(M) of 2.21 A(3)Da(-1) and a solvent content of 44.3%.

摘要

β-木糖苷酶参与木聚糖分解为木糖的过程,属于糖基水解酶家族39或43。目前,这些家族的任何成员均无结构信息。来自嗜热厌氧菌嗜糖栖热厌氧菌的β-木糖苷酶是糖基水解酶家族39的成员,已采用悬滴气相扩散法在296 K下结晶。该晶体在同步加速器X射线下衍射分辨率为2.4 Å,属于空间群P4(1)2(1)2(或P4(3)2(1)2),晶胞参数a = b = 92.75,c = 241.37 Å。不对称单元包含重组酶的两个单体,相应的V(M)为2.21 Å3Da-1,溶剂含量为44.3%。

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