[鼻咽癌及人正常鼻咽组织中p53调控基因差异表达谱的cDNA表达阵列分析]
[cDNA expression array in the differential expression profiles of p53 regulated genes in nasopharyngeal carcinoma and the human normal nasopharynx].
作者信息
Li H, Han W, Zhang L
机构信息
Carcinogenesis Key Laboratory of Ministry of Public Health, Cancer Institute, Central Southern University, Changsha 410078, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2001 Nov;23(6):448-50.
OBJECTIVE
To compare the gene expression map of nasopharyngeal carcinoma (NPC) tissue with that of the control tissue by cDNA array and to discuss possible reasons of TP53 accumulation in NPC tissue.
METHODS
After the hybridization of Atlas human cancer cDNA expression array 7742-1, analysis of Atlas arrays by means of AtlasImage 1.01a was carried out. Then, the results of array were verified by reverse transcription-polymerase chain reaction (RT-PCR). Gene expression alteration on the protein level was verified by immunohistochemistry.
RESULTS
134 of 588 tumor-related genes were upregulated and 88 downregulated. Thirteen of 32 p53-regulated genes showed differential expression with 11 upregulated and 2 downregulated.
CONCLUSION
(1) p53 dysfunction exists in NPC tissues, (2) MDM2, p21 and Bax may be involved in the regulation of nasopharyngeal carcinoma cell growth.
目的
通过cDNA阵列比较鼻咽癌（NPC）组织与对照组织的基因表达图谱，并探讨NPC组织中TP53积累的可能原因。
方法
对Atlas人类癌症cDNA表达阵列7742 - 1进行杂交后，使用AtlasImage 1.01a对Atlas阵列进行分析。然后，通过逆转录 - 聚合酶链反应（RT-PCR）验证阵列结果。通过免疫组织化学验证蛋白质水平上的基因表达改变。
结果
588个肿瘤相关基因中有134个上调，88个下调。32个p53调节基因中有13个显示差异表达，其中11个上调，2个下调。
结论
（1）NPC组织中存在p53功能障碍，（2）MDM2、p21和Bax可能参与鼻咽癌细胞生长的调节。
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