[利用多色荧光原位杂交技术研究秋水仙碱和环磷酰胺诱导的微核]
[Studies on micronuclei induced by colchicine and cyclophosphamide using multicolor fluorescence in situ hybridization].
作者信息
Yang M, Cao J
机构信息
Molecular Toxicology Labortory, Third Military University, Chongqing 400038, China.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 2000 Mar;34(2):101-3.
OBJECTIVE
To study chromosomal composition of micronuclei (MN) induced by colchicine (COL) and cyclophosphamide (CP) in mouse bone marrow erythrocytes.
METHODS
Multicolor fluorescence in situ hybridization (FISH) with centromeric and telomeric DNA probes was applied to analyze chromosomal composition of micronuclei induced by COL and CP in mouse bone marrow erythrocytes.
RESULTS
About 83.5% of COL-induced MN revealed both centromeric and telomeric signals. Of the CP-induced MN, 74.5% showed telomeric signals only.
CONCLUSION
Majority of COL-induced MN contain whole chromosome and that of CP-induced MN mainly contain acentric fragments. Multicolor FISH with centromeric and telomeric DNA probes was a precise technique for analyzing chromosomal composition of MN.
目的
研究秋水仙碱（COL）和环磷酰胺（CP）诱导的小鼠骨髓红细胞微核（MN）的染色体组成。
方法
应用着丝粒和端粒DNA探针的多色荧光原位杂交（FISH）技术分析COL和CP诱导的小鼠骨髓红细胞微核的染色体组成。
结果
约83.5%的COL诱导微核同时显示着丝粒和端粒信号。在CP诱导的微核中，74.5%仅显示端粒信号。
结论
COL诱导的微核多数包含整条染色体，而CP诱导的微核主要包含无着丝粒片段。着丝粒和端粒DNA探针的多色FISH技术是分析微核染色体组成的精确技术。
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