Jie Y M, Jia C
Molecular Toxicology Laboratory, Third Military Medical University, Chongqing 400038, China.
Mutagenesis. 2001 Mar;16(2):145-9. doi: 10.1093/mutage/16.2.145.
The chromosomal composition of micronuclei (MN) induced by the model mutagens mitomycin (MMC) and colchicine (COL) as well as by acrylamide (AA) and the traditional Chinese medicine Tripterygium hypoglaucum (level) hutch (THH) in NIH 3T3 cells was analyzed by multicolor fluorescence in situ hybridization (FISH) using DNA probes for the centromere repeated minor satellite DNA and the telomeric hexamer repeat (TTAGGG). The majority of MN (78.6%) from treatment with MMC (0.1 microg/ml) did not show centromeric signals, reflecting the clastogenic action of MMC. Following treatment with COL (0.1 microg/ml), 74.5% of the MN showed centromeric signals and several telomeric signals, indicating that MN induced by this well-known aneugen were mainly composed of whole chromosomes. After treatment with AA (100, 200 and 400 microg/ml) both MN containing whole chromosomes and MN containing acentric fragments were found to increase in a dose-dependent manner, demonstrating that AA is not only a clastogen but also an aneugen. THH induced a high frequency of MN harboring whole chromosomes at all concentrations tested (5, 10 and 20 microl/ml) and produced a dose-dependent increase in fragment-containing MN, indicating that THH has both aneugenic and clastogenic potential.
利用针对着丝粒重复小卫星DNA和端粒六聚体重复序列（TTAGGG）的DNA探针，通过多色荧光原位杂交（FISH）分析了模型诱变剂丝裂霉素（MMC）、秋水仙碱（COL）、丙烯酰胺（AA）以及中药青藤碱（THH）在NIH 3T3细胞中诱导产生的微核（MN）的染色体组成。用MMC（0.1微克/毫升）处理后，大多数微核（78.6%）未显示着丝粒信号，这反映了MMC的断裂作用。用COL（0.1微克/毫升）处理后，74.5%的微核显示着丝粒信号和几个端粒信号，表明这种著名的非整倍体诱导剂诱导产生的微核主要由整条染色体组成。用AA（100、200和400微克/毫升）处理后，发现含有整条染色体的微核和含有无着丝粒片段的微核均呈剂量依赖性增加，这表明AA不仅是一种断裂剂，也是一种非整倍体诱导剂。在所有测试浓度（5、10和20微升/毫升）下，THH诱导产生的微核中含有整条染色体的频率很高，并且含片段微核呈剂量依赖性增加，这表明THH具有非整倍体诱导和断裂的潜力。
