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1-甲基腺苷化学掺入寡核苷酸。

Chemical incorporation of 1-methyladenosine into oligonucleotides.

作者信息

Mikhailov Sergey N, Rozenski Jef, Efimtseva Ekaterina V, Busson Roger, Van Aerschot Arthur, Herdewijn Piet

机构信息

Laboratory of Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium.

出版信息

Nucleic Acids Res. 2002 Mar 1;30(5):1124-31. doi: 10.1093/nar/30.5.1124.

DOI:10.1093/nar/30.5.1124
PMID:11861902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC101250/
Abstract

The base moiety of 1-N-methyladenosine can be protected with a chloroacetyl group for incorporation of this modified nucleoside into DNA and RNA. Carefully controlled anhydrous conditions are needed for deprotection of the oligonucleotides.

摘要

1-N-甲基腺苷的碱基部分可用氯乙酰基保护，以便将这种修饰的核苷掺入DNA和RNA中。寡核苷酸的脱保护需要严格控制无水条件。

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本文引用的文献

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Analysis of Oligonucleotides by HPLC-Electrospray Ionization Mass Spectrometry.高效液相色谱-电喷雾电离质谱法分析寡核苷酸
Anal Chem. 1997 Apr 1;69(7):1320-5. doi: 10.1021/ac960916h.
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Synthesis and properties of O-beta-D-ribofuranosyl-(1"-2')-adenosine-5"-O-phosphate and its derivatives.
Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1847-59. doi: 10.1080/15257770008045465.
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Base-modified phosphoramidite analogs of pyrimidine ribonucleosides for RNA structure-activity studies.用于RNA结构活性研究的嘧啶核糖核苷的碱基修饰亚磷酰胺类似物。
Methods Enzymol. 2000;317:39-65. doi: 10.1016/s0076-6879(00)17005-3.
4
The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae.Gcd10p/Gcd14p复合物是酿酒酵母中必不可少的双亚基tRNA（1-甲基腺苷）甲基转移酶。
Proc Natl Acad Sci U S A. 2000 May 9;97(10):5173-8. doi: 10.1073/pnas.090102597.
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A Watson-Crick base-pair-disrupting methyl group (m1A9) is sufficient for cloverleaf folding of human mitochondrial tRNALys.一个破坏沃森-克里克碱基对的甲基基团（m1A9）足以使人线粒体赖氨酸转运RNA（tRNALys）形成三叶草折叠结构。
Biochemistry. 1999 Oct 5;38(40):13338-46. doi: 10.1021/bi991061g.
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The RNA Modification Database: 1999 update.RNA修饰数据库：1999年更新版。
Nucleic Acids Res. 1999 Jan 1;27(1):196-7. doi: 10.1093/nar/27.1.196.
7
The presence of modified nucleotides is required for cloverleaf folding of a human mitochondrial tRNA.人线粒体tRNA的三叶草折叠需要修饰核苷酸的存在。
Nucleic Acids Res. 1998 Apr 1;26(7):1636-43. doi: 10.1093/nar/26.7.1636.
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Compilation of tRNA sequences and sequences of tRNA genes.转运RNA序列及转运RNA基因序列的汇编。
Nucleic Acids Res. 1998 Jan 1;26(1):148-53. doi: 10.1093/nar/26.1.148.
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The importance of being modified: roles of modified nucleosides and Mg2+ in RNA structure and function.修饰的重要性：修饰核苷和Mg2+在RNA结构与功能中的作用
Prog Nucleic Acid Res Mol Biol. 1996;53:79-129. doi: 10.1016/s0079-6603(08)60143-9.
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The primary structure of cytoplasmic initiator tRNA(Met) from Schizosaccharomyces pombe.粟酒裂殖酵母胞质起始tRNA（Met）的一级结构。
Nucleic Acids Res. 1993 Jun 25;21(12):2949. doi: 10.1093/nar/21.12.2949.