论大脑中神经递质摄取与释放的功能耦合。
On the functional coupling of neurotransmitter uptake and release in brain.
作者信息
Cotman C W, Haycock J W, Levy W B
出版信息
Br J Pharmacol. 1976 Dec;58(4):569-72. doi: 10.1111/j.1476-5381.1976.tb08625.x.
1 Isolated synaptosomal fractions from mouse forebrains were incubated [14C]-gamma-aminobutyric acid ([14C]-GABA). Release of the accumulated label in high potassium solution was measured. 2 The fractional release dependent upon calcium was decreased by raising the concentration of [14C]-GABA during labeling but was not affected by altering the time allowed for labelling or the time between labelling and stimulation. 3 These data suggest that extracellular GABA gains rapid access to available intraterminal pools. The relative distribution of the accumulated GABA in differerent pools can be influenced by the concentration of GABA in the incubation medium but, once (stored", there is no net redistribution of accumulated GABA in the absence of stimulation.
从小鼠前脑分离出的突触体组分与[14C] -γ-氨基丁酸（[14C] -GABA）一起孵育。测量在高钾溶液中积累的标记物的释放情况。
在标记过程中提高[14C] -GABA的浓度会降低依赖于钙的分数释放，但改变标记时间或标记与刺激之间的时间不受影响。
这些数据表明细胞外GABA可快速进入可用的终末内池。孵育培养基中GABA的浓度可影响积累的GABA在不同池中的相对分布，但是，一旦（储存），在没有刺激的情况下积累的GABA不会有净再分布。
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