谷胱甘肽-S-转移酶μ和θ基因多态性作为特应性支气管哮喘的新危险因素

Glutathione- S-transferase micro and theta gene polymorphisms as new risk factors of atopic bronchial asthma.

作者信息

Ivaschenko T E, Sideleva O G, Baranov V S

机构信息

Institute of Obstetrics and Gynecology, 199034 Mendeleevskaya line 3, St. Petersburg, Russia.

出版信息

J Mol Med (Berl). 2002 Jan;80(1):39-43. doi: 10.1007/s001090100274. Epub 2001 Sep 6.

DOI:10.1007/s001090100274

PMID:11862323

Abstract

The genetic polymorphism of glutathione- S-transferase M1 (GSTM1) and glutathione- S-transferase T1 (GSTT1) genes and the cytochrome P4501A1 gene responsible for xenobiotic conjugating enzymes of the phase II and phase I detoxification system were studied by PCR-RFLP in the blood spots of 109 patients with atopic bronchial asthma and 90 healthy individuals. GSTM1 gene deletion (GSTM1(0/0)) was detected in 47.8% of individuals in the control group and in 76.1% of asthmatic patients. Individuals without the GSTM1 gene were at approximately 3.5--fold higher risk of developing asthma. The proportion of GSTT1(0/0) genotypes was significantly higher in the group of asthmatics (67.0%) than in controls (23.3%). The proportion of individuals with a deficiency in both GSTM1 and GSTT1 gene activity was more than four times higher in asthmatic patients than in the control group (54.1% and 12.2%, respectively). The frequency of the Ile-Val polymorphism of the CYP1A1 gene was similar in controls and asthmatic patients. This study shows the association of atopic bronchial asthma with GSTM1(0/0), GSTT1(0/0) genotypes.

摘要

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对109例特应性支气管哮喘患者和90名健康个体血斑中谷胱甘肽S-转移酶M1（GSTM1）、谷胱甘肽S-转移酶T1（GSTT1）基因以及负责Ⅰ、Ⅱ相解毒系统外源性物质结合酶的细胞色素P4501A1（CYP1A1）基因的遗传多态性进行了研究。对照组中47.8%的个体检测到GSTM1基因缺失（GSTM1(0/0)），哮喘患者中这一比例为76.1%。无GSTM1基因的个体患哮喘的风险约高3.5倍。哮喘组中GSTT1(0/0)基因型的比例（67.0%）显著高于对照组（23.3%）。GSTM1和GSTT1基因活性均缺乏的个体比例在哮喘患者中比对照组高出四倍多（分别为54.1%和12.2%）。CYP1A1基因Ile-Val多态性的频率在对照组和哮喘患者中相似。本研究显示特应性支气管哮喘与GSTM1(0/0)、GSTT1(0/0)基因型有关。