Halbhuber K J, Christner A, Geyer G
Acta Histochem. 1979;65(1):70-4.
The present study applies semithin sections for electron microscopic autoradiography. Due to (a) the decrease of exposition time, and (b) lower intensity of isotope labelling, low power electron microscopic autoradiography on semithin sections is favoured in comparison with conventional (ultrathin section high power) electron microscopic autoradiography, and due to (c) more precise localization of silver granules, and (d) ease of quantitative evaluation of autoradiography, it is superior to ordinary (thick section) light microscopic autoradiography.
本研究将半薄切片应用于电子显微镜放射自显影。由于(a)曝光时间的减少,以及(b)同位素标记强度较低,与传统的(超薄切片高倍)电子显微镜放射自显影相比,半薄切片的低倍电子显微镜放射自显影更受青睐,并且由于(c)银颗粒的定位更精确,以及(d)放射自显影定量评估更容易,它优于普通的(厚切片)光学显微镜放射自显影。