Ma A, Duthie S J, Ross M A, Collins A R
Department of Human Nutrition, Qingdao Medical College, Qingdao, Shandong 266021.
Zhonghua Yu Fang Yi Xue Za Zhi. 1999 Jan;33(1):16-7.
To investigate their effects on lymphocyte damage with supplementation of high-dose vitamins E, C and beta-carotene.
Healthy men aged 50 - 59 years were selected and randomized into the trial and control groups with 50 in each one. In the trial group, 25 mg of beta-carotene, 100 mg vitamin C and 280 mg vitamin E were given to each subject every day for 20 weeks. Whole blood was collected in both the trial and control groups and its lymphocytes separated. Damage to DNA was analyzed with a"comet" electrophoresis technique, and serum levels of vitamin C, beta-carotene and alpha-tocopherol were determined by high performance liquid chromatography.
There was no significant difference in the proportions of spontaneous damage to DNA in lymphocytes between the trial and control groups (6.1% vs. 6.8%). But, proportions of damage to DNA in peripheral lymphocytes increased to 36.14%, 59.45, and 69.62%, respectively, after treatment with 30 micromol/L, 100 micromol/L and 300 micromol/L of H(2)O(2).
Supplementation of vitamin E, C and beta-carotene could effectively reduce the damage to DNA caused by H(2)O(2).
研究补充大剂量维生素E、C和β-胡萝卜素对淋巴细胞损伤的影响。
选取50 - 59岁的健康男性，随机分为试验组和对照组，每组50人。试验组中，每位受试者每天给予25毫克β-胡萝卜素、100毫克维生素C和280毫克维生素E，持续20周。试验组和对照组均采集全血并分离淋巴细胞。采用“彗星”电泳技术分析DNA损伤情况，通过高效液相色谱法测定血清中维生素C、β-胡萝卜素和α-生育酚的水平。
试验组和对照组淋巴细胞DNA自发损伤比例无显著差异（6.1%对6.8%）。但是，用30微摩尔/升、100微摩尔/升和300微摩尔/升的过氧化氢处理后，外周淋巴细胞DNA损伤比例分别增至36.14%、59.45%和69.62%。
补充维生素E、C和β-胡萝卜素可有效减轻过氧化氢所致的DNA损伤。
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