[营养不良新生大鼠胰岛素、胰高血糖素及胰岛素样生长因子-II基因表达的变化]
[Changes in expression of genes for insulin, glucagon and IGF-II in neonatal rats with malnutrition].
作者信息
Li Q, Bréat B, Czernichow P
机构信息
Department of Endocrinology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016.
出版信息
Zhonghua Yu Fang Yi Xue Za Zhi. 1999 Jul;33(4):203-5.
OBJECTIVE
To study the effects of malnutrition on the function of cells in fetal islet of pancreas.
METHODS
A neonatal rat model with malnutrition was prepared by maternal food restriction (fed with less than half amount of the normal) during the 14th to 21st days of pregnancy. Expression of genes for insulin and glucagon in the pancreas, as well as expression of genes for insulin-like growth factor II (IGF-II) in the pancreas and liver of the rats were analyzed with Northern blotting technique.
RESULTS
Pancreatic content of insulin mRNA was significantly lower in neonatal rats with malnutrition than that in the normal group (P < 0.05), but no obvious changes in glucagon mRNA in the pancreas was found. There was no significant difference in IGF-II mRNA content in the pancreas and liver between the neonatal rats with malnutrition and the normal control group.
CONCLUSION
Malnutrition during rat fetal development could cause inhibition of expression of insulin gene, but no obvious effects on the expression of glucagon and IGF-II genes.
目的
研究营养不良对胎儿胰岛细胞功能的影响。
方法
通过在妊娠第14至21天对孕鼠进行食物限制（喂食量少于正常量的一半）制备营养不良的新生大鼠模型。采用Northern印迹技术分析大鼠胰腺中胰岛素和胰高血糖素基因的表达，以及大鼠胰腺和肝脏中胰岛素样生长因子II（IGF-II）基因的表达。
结果
营养不良新生大鼠胰腺中胰岛素mRNA含量显著低于正常组（P < 0.05），但胰腺中胰高血糖素mRNA未发现明显变化。营养不良新生大鼠与正常对照组之间胰腺和肝脏中IGF-II mRNA含量无显著差异。
结论
大鼠胎儿发育期间的营养不良可导致胰岛素基因表达受到抑制，但对胰高血糖素和IGF-II基因的表达无明显影响。
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