Kitago Kuniaki, Ichikawa Wataru, Uetake Hiroyuki, Nihei Zenro
Second Dept. of Surgery, Saitama Medical School.
Gan To Kagaku Ryoho. 2002 Feb;29(2):245-52.
The rate-limiting enzyme to catabolize 5-fluorouracil (5-FU) is dihydropyrimidine dehydrogenase (DPD), which expression in cancerous tissue is reported to have a relation with anti-tumor effect for 5-FU. In this study, we evaluated the immunohistochemical expression in gastric cancer using two different kinds of anti-human DPD antibody, KM1915 and KM1919. However, recognition of both antibody was mimetic in human gastric cancer, and strong expression of DPD was observed in the interstitial tissue when using KM1919. There is a positive relationship between immunohistochemical expression by KM1915 and mRNA expression in human gastric cancer. Immunohistochemical study with KM1915 might be a clinically useful method to evaluate the expression of DPD in paraffin-embedded materials.
分解代谢5-氟尿嘧啶（5-FU）的限速酶是二氢嘧啶脱氢酶（DPD），据报道其在癌组织中的表达与5-FU的抗肿瘤作用有关。在本研究中，我们使用两种不同的抗人DPD抗体KM1915和KM1919评估了胃癌中的免疫组化表达。然而，在人胃癌中这两种抗体的识别情况相似，使用KM1919时在间质组织中观察到DPD的强表达。KM1915的免疫组化表达与人胃癌中的mRNA表达呈正相关。用KM1915进行免疫组化研究可能是评估石蜡包埋材料中DPD表达的一种临床有用方法。
