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一种秀丽隐杆线虫的转化生长因子β（TGF-β），即DBL-1，控制LON-1（一种与PR相关的蛋白质）的表达，LON-1可调节多倍体化和体长。

A Caenorhabditis elegans TGF-beta, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length.

作者信息

Morita Kiyokazu, Flemming Anthony J, Sugihara Yukiko, Mochii Makoto, Suzuki Yo, Yoshida Satoru, Wood William B, Kohara Yuji, Leroi Armand M, Ueno Naoto

机构信息

Department of Developmental Biology, National Institute for Basic Biology, The Graduate University for Advanced Studies, Okazaki 444-8585, Japan.

出版信息

EMBO J. 2002 Mar 1;21(5):1063-73. doi: 10.1093/emboj/21.5.1063.

DOI:10.1093/emboj/21.5.1063
PMID:11867534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC125886/
Abstract

Using cDNA-based array analysis combined with double-stranded RNA interference (dsRNAi), we have identified yk298h6 as a target gene of Caenorhabditis elegans TGF-beta signaling. Worms overexpressing dbl-1, a TGF-beta ligand, are 16% longer than wild type. Array analysis shows yk298h6 to be one of several genes suppressed in such worms. Disruption of yk298h6 function by dsRNAi also resulted in long worms, suggesting that it is a negative regulator of body length. yk298h6 was then mapped to, and shown to be identical to, lon-1, a known gene that affects body length. lon-1 encodes a 312 amino acid protein with a motif sequence that is conserved from plants to humans. Expression studies confirm that LON-1 is repressed by DBL-1, suggesting that LON-1 is a novel downstream component of the C.elegans TGF-beta growth regulation pathway. Consistent with this, LON-1 is expressed mainly in the larval and adult hypodermis and has dose-dependent effects on body length associated with changes in hypodermal ploidy, but not hypodermal cell proliferation.

摘要

通过基于cDNA的阵列分析结合双链RNA干扰（dsRNAi），我们已将yk298h6鉴定为秀丽隐杆线虫TGF-β信号传导的一个靶基因。过表达TGF-β配体dbl-1的线虫比野生型长16%。阵列分析表明yk298h6是此类线虫中被抑制的几个基因之一。通过dsRNAi破坏yk298h6的功能也导致线虫体长增加，这表明它是体长的负调节因子。然后，yk298h6被定位到并被证明与lon-1相同，lon-1是一个已知的影响体长的基因。lon-1编码一种含有312个氨基酸的蛋白质，其基序序列从植物到人类都保守。表达研究证实LON-1受DBL-1抑制，这表明LON-1是秀丽隐杆线虫TGF-β生长调节途径的一个新的下游成分。与此一致的是，LON-1主要在幼虫和成虫的皮下组织表达，并且对与皮下多倍体变化相关的体长有剂量依赖性影响，但对皮下细胞增殖没有影响。

相似文献

[1]
A Caenorhabditis elegans TGF-beta, DBL-1, controls the expression of LON-1, a PR-related protein, that regulates polyploidization and body length.

EMBO J. 2002-3-1

[2]
lon-1 regulates Caenorhabditis elegans body size downstream of the dbl-1 TGF beta signaling pathway.

Dev Biol. 2002-6-15

[3]
Increased or decreased levels of Caenorhabditis elegans lon-3, a gene encoding a collagen, cause reciprocal changes in body length.

Genetics. 2002-5

[4]
A cuticle collagen encoded by the lon-3 gene may be a target of TGF-beta signaling in determining Caenorhabditis elegans body shape.

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[5]
The expression of TGFbeta signal transducers in the hypodermis regulates body size in C. elegans.

Development. 2002-11

[6]
Hypodermal expression of Caenorhabditis elegans TGF-beta type I receptor SMA-6 is essential for the growth and maintenance of body length.

Dev Biol. 2001-12-1

[7]
C. elegans serine-threonine kinase KIN-29 modulates TGFbeta signaling and regulates body size formation.

BMC Dev Biol. 2005-4-19

[8]
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[9]
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[10]
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[3]
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[5]
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[6]
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[7]
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[8]
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Parasit Vectors. 2023-8-17

[9]
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[10]
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本文引用的文献

[1]
Increased or decreased levels of Caenorhabditis elegans lon-3, a gene encoding a collagen, cause reciprocal changes in body length.

Genetics. 2002-5

[2]
A novel mode of ecdysozoan growth in Caenorhabditis elegans.

Evol Dev. 2002

[3]
Hypodermal expression of Caenorhabditis elegans TGF-beta type I receptor SMA-6 is essential for the growth and maintenance of body length.

Dev Biol. 2001-12-1

[4]
Cell cycle activation by plant parasitic nematodes.

Plant Mol Biol. 2000-8

[5]
A global profile of germline gene expression in C. elegans.

Mol Cell. 2000-9

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Biomedical discovery with DNA arrays.

Cell. 2000-7-7

[7]
A Caenorhabditis elegans type I TGF beta receptor can function in the absence of type II kinase to promote larval development.

Development. 2000-8

[8]
Genomics, gene expression and DNA arrays.

Nature. 2000-6-15

[9]
Measuring dimensions: the regulation of size and shape.

Development. 2000-7

[10]
Double-stranded RNA as a template for gene silencing.

Cell. 2000-4-28

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