增殖细胞核抗原在儿童肾母细胞瘤中的预后价值

Prognostic value of proliferating cell nuclear antigen in Wilms' tumour in children.

作者信息

Skotnicka-Klonowicz G, Kobos J, Łoś E, Trejster E, Szymik-Kantorowicz S, Daszkiewicz P

机构信息

Clinic of Surgery and Paediatric Oncology, II Clinic of Children's Diseases, Institute of Paediatrics, Medical University of Lodz, ul. Sporna 36/50, 91-738 Lodz, Poland.

出版信息

Eur J Surg Oncol. 2002 Feb;28(1):67-71. doi: 10.1053/ejso.2001.1208.

DOI:10.1053/ejso.2001.1208

PMID:11869017

Abstract

AIMS

To evaluate the prognostic value of index Proliferating Cell Nuclear Antigen (PCNA) in Wilms' tumour in children.

METHODS

The study comprised 64 children aged from 2 days to 13 years treated according to the SIOP (Society International of Oncology Paediatric) and accepted by the PPGGL (Polish Paediatric Group for the Treatment of Solid Tumours). The studies were conducted on tumour tissue removed during surgery, fixed in formalin and embedded in paraffin blocks. Sections (4 microns) were evaluated by immunohistochemistry, using the peroxidase method to determine the expression of PCNA in Wilms' tumour cells by primary monoclonal antibody NCL-PCNA from Novocastra.

RESULTS

The percentage of immunopositive cells in particular fragments of the tumour ranged from 0--93%, mean 30.5%, median 25.5%. Mean and median values enabled division of children into two groups: Group A, where the percentage of cells staining with anti-PCNA was <30% and Group B, where this percentage was >30%. The expression of PCNA was evaluated in various stages of advancement, various histological types and depending on the course of disease. The studies revealed the correlation between index PCNA and stage of advancement P<0.01, index PCNA and histological type of Wilms' tumour P<0.025. Moreover we observed that deaths were found more frequently in tumours with index PCNA >30%, P<0.001.

CONCLUSIONS

PCNA is a useful prognostic factor in Wilms' tumour in children.

摘要

目的

评估增殖细胞核抗原（PCNA）指数在儿童肾母细胞瘤中的预后价值。

方法

该研究纳入了64名年龄从2天至13岁的儿童，他们均按照国际小儿肿瘤学会（SIOP）的方案接受治疗，并被波兰实体肿瘤治疗小儿小组（PPGGL）所接受。研究对象为手术切除的肿瘤组织，用福尔马林固定并包埋于石蜡块中。采用免疫组织化学方法，运用过氧化物酶法，以诺华卡斯托公司的鼠抗人PCNA单克隆抗体NCL-PCNA检测肾母细胞瘤细胞中PCNA的表达，对4微米厚的切片进行评估。

结果

肿瘤特定片段中免疫阳性细胞的百分比范围为0% - 93%，平均为30.5%，中位数为25.5%。根据平均值和中位数可将儿童分为两组：A组，抗PCNA染色细胞百分比<30%；B组，该百分比>30%。对不同进展阶段、不同组织学类型以及疾病进程中PCNA的表达进行了评估。研究发现PCNA指数与进展期之间存在相关性（P<0.01），PCNA指数与肾母细胞瘤的组织学类型之间存在相关性（P<0.025）。此外，我们观察到PCNA指数>30%的肿瘤中死亡更为常见（P<0.001）。