Peng Xuanxian, Zhang Jianying, Wang Sanying, Lin Zhilin, Zhang Weiyi
Department of Biology and the Key Laboratory of Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, PR China.
J Microbiol Methods. 2002 May;49(3):335-8. doi: 10.1016/s0167-7012(02)00010-6.
In this report, we describe the use of universal primer PCR (UPPCR) for the detection of 16S ribosomal RNA (rRNA) genes from Aeromonas hydrophila captured by anti-A. hydrophila antibody coupled to a microplate. The approach combining immuno-capture with UPPCR provides a quick, sensitive, and reproducible way for the detection of bacterial cells.
在本报告中，我们描述了使用通用引物PCR（UPPCR）来检测与微孔板偶联的抗嗜水气单胞菌抗体捕获的嗜水气单胞菌的16S核糖体RNA（rRNA）基因。免疫捕获与UPPCR相结合的方法为细菌细胞的检测提供了一种快速、灵敏且可重复的方式。
