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在肠化生进展过程中,同源框基因CDX2的表达先于CDX1。

Expression of homeobox gene CDX2 precedes that of CDX1 during the progression of intestinal metaplasia.

作者信息

Eda Akashi, Osawa Hiroyuki, Yanaka Ichiro, Satoh Kiichi, Mutoh Hiroyuki, Kihira Ken, Sugano Kentaro

机构信息

Department of Gastroenterology, Jichi Medical School, Kawachi, Tochigi, Japan.

出版信息

J Gastroenterol. 2002;37(2):94-100. doi: 10.1007/s005350200002.

Abstract

BACKGROUND

The CDX1 and CDX2 genes are intestinal transcription factors that may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. There have been no detailed reports directly comparing the expression of CDX1 with that of CDX2 in chronic gastritis and intestinal metaplasia. Accordingly, we examined the expression of CDX1/2 and its association with the expression of other intestinal metaplasia-associated genes during the development of intestinal metaplasia.

METHODS

The expression of CDX1/2 genes was analyzed, using the reverse transcriptase-polymerase chain reaction, in 44 human gastric tissue samples obtained endoscopically. The expressions of mucin markers (MUC2, MUC5AC), intestine-specific genes (sucrase-isomaltase, human defensin-5, alkaline phosphatase), a gene marker for fundic gland area (H+/K+ATPase beta subunit), and a gene for entire gastric glands (pepsinogen C) were also comparatively analyzed.

RESULTS

There was no expression of CDX1/2 in gastric mucosa not infected by Helicobacter pylori. The prevalence of CDX1 mRNA expression was significantly higher in mucosa with intestinal metaplasia than in mucosa without intestinal metaplasia. It is noteworthy that CDX2 was expressed in the antral and fundic mucosa in the absence of the expression of CDX1 and gene markers for intestinal metaplasia.

CONCLUSIONS

The expression of CDX2 precedes those of CDX1, sucrase-isomaltase, other intestine-specific genes (human defensin-5, alkaline phosphatase), and MUC2 during the progression of intestinal metaplasia. These findings imply that the expression of CDX2 may trigger the initiation and development of intestinal metaplasia.

摘要

背景

CDX1和CDX2基因是肠道转录因子,可能参与肠道上皮细胞增殖和分化的调控。目前尚无直接比较慢性胃炎和肠化生中CDX1与CDX2表达的详细报道。因此,我们研究了肠化生发展过程中CDX1/2的表达及其与其他肠化生相关基因表达的关系。

方法

采用逆转录-聚合酶链反应分析44例经内镜获取的人胃组织样本中CDX1/2基因的表达。同时比较分析黏蛋白标志物(MUC2、MUC5AC)、肠道特异性基因(蔗糖酶-异麦芽糖酶、人防御素-5、碱性磷酸酶)、胃底腺区基因标志物(H+/K+ATP酶β亚基)和全胃腺基因(胃蛋白酶原C)的表达。

结果

未感染幽门螺杆菌的胃黏膜中无CDX1/2表达。肠化生黏膜中CDX1 mRNA表达的发生率显著高于无肠化生的黏膜。值得注意的是,在没有CDX1和肠化生基因标志物表达的情况下,CDX2在胃窦和胃底黏膜中表达。

结论

在肠化生进展过程中,CDX2的表达先于CDX1、蔗糖酶-异麦芽糖酶、其他肠道特异性基因(人防御素-5、碱性磷酸酶)和MUC2。这些发现提示CDX2的表达可能触发肠化生的起始和发展。

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