For over three decades, the technique of microneurography has allowed us to record sympathetic neural outflow directly from postganglionic axons in awake human subjects. But because sympathetic axons are clustered within a nerve fascicle, such recordings have been limited to the analysis of multi-unit neural activity. To improve the information content of intraneural recordings, we developed the single-unit approach, in which focal recordings can be made from a single C-fibre via a high-impedance tungsten microelectrode. In this review, we describe our methodology for analyzing unitary sympathetic activity and discuss the similarities in the firing properties of individual muscle vasoconstrictor, cutaneous vasoconstrictor and sudomotor neurones.