不动杆菌属菌株M-1利用正构烷烃生产蜡酯：细胞内含物的超微结构及酰基辅酶A还原酶的作用
Wax ester production from n-alkanes by Acinetobacter sp. strain M-1: ultrastructure of cellular inclusions and role of acyl coenzyme A reductase.
作者信息
Ishige Takeru, Tani Akio, Takabe Keiji, Kawasaki Kazunori, Sakai Yasuyoshi, Kato Nobuo
机构信息
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan.
出版信息
Appl Environ Microbiol. 2002 Mar;68(3):1192-5. doi: 10.1128/AEM.68.3.1192-1195.2002.
Abstract
Acinetobacter sp. strain M-1 accumulated a large amount of wax esters from an n-alkane under nitrogen-limiting conditions. Under the optimized conditions with n-hexadecane as the substrate, the amount of hexadecyl hexadecanoate in the cells reached 0.17 g/g of cells (dry weight). Electron microscopic analysis revealed that multilayered disk-shaped intracellular inclusions were formed concomitant with wax ester formation. The contribution of acyl-CoA reductase to wax ester synthesis was evaluated by gene disruption analysis.
摘要
不动杆菌属菌株M-1在氮限制条件下从正构烷烃中积累了大量蜡酯。在以正十六烷为底物的优化条件下，细胞中十六烷基十六烷酸酯的含量达到0.17 g/g细胞（干重）。电子显微镜分析表明，多层盘状细胞内包涵体是伴随蜡酯形成而形成的。通过基因敲除分析评估了酰基辅酶A还原酶对蜡酯合成的贡献。
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