Wang Q, Wu Z
Department of Rehibilitation Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038 P. R. China.
Zhonghua Shao Shang Za Zhi. 2001 Feb;17(1):25-8.
To investigate the relationships between the development of hypertrophic scar (HS) and apoptosis of fibroblast and endothelial cell, and to elucidate the influence of related modulating factors.
TUNEL and immunohistochemistry staining techniques were employed to examine apoptosis of fibroblast and endothelial cell. The expression of ICE and Bcl-2 in hypertrophic scar of 61 cases of postburn patients undergoing plastic surgery with HS and in 20 other operative patients with non-hypertrophic scars (NHS) was also determined.
The positive apoptotic cell indices of fibroblast and vascular endothelial cells during the proliferative stage of HS were 6.60 plus minus 4.43 and 8.90 plus minus 6.01 respectively, while those during the mature stage were 25.60 plus minus 5.70 and and 26.6 plus minus 6.02, respectively. There existed evident differences in positive apoptotic cell indices between the same kind of cells (fibroblasts or vascular endothelial cells) during the two stage (P < 0.01). The ICE positive expression rate in proliferative stage was much lower than that in mature stage (P < 0.01), but the Bcl-2 positive expression rate in proliferative stage was obviously higher than that in mature stage (P < 0.01).
HS development might be related to the decrement of apoptotic fibroblasts and endothelial cells. ICE and Bcl-2 were possibly involved in the modulation of fibroblast and endothelial cell apoptosis in HS.
探讨增生性瘢痕（HS）的发生发展与成纤维细胞及内皮细胞凋亡的关系，并阐明相关调节因子的影响。
采用TUNEL和免疫组化染色技术检测成纤维细胞和内皮细胞凋亡情况。同时检测61例烧伤后行整形手术的增生性瘢痕患者及20例其他手术的非增生性瘢痕（NHS）患者增生性瘢痕中ICE和Bcl-2的表达。
HS增殖期成纤维细胞和血管内皮细胞的阳性凋亡细胞指数分别为6.60±4.43和8.90±6.01，而成熟期分别为25.60±5.70和26.6±6.02。同一类细胞（成纤维细胞或血管内皮细胞）在两个阶段的阳性凋亡细胞指数存在明显差异（P<0.01）。增殖期ICE阳性表达率明显低于成熟期（P<0.01），但增殖期Bcl-2阳性表达率明显高于成熟期（P<0.01）。
HS的发生发展可能与成纤维细胞和内皮细胞凋亡减少有关。ICE和Bcl-2可能参与了HS中成纤维细胞和内皮细胞凋亡的调节。
