Chen Jing, Wang Jia-han, Zhuang Hong-xing
Department of Burns, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China.
Zhonghua Shao Shang Za Zhi. 2006 Aug;22(4):277-80.
To further explore the effects of substance P on the proliferation and apoptosis of fibroblasts obtained from pathological scars in vitro.
Fibroblasts from keloid (KSF) , hypertrophic scar (HSF) and normal dermis (NDF) of 12 burn patients were cultured in vitro and divided into control, SP (with 1 x 10 (-6) mol/L SP added to the culture medium) , and SP + spantide( with 1 x 10 (-6) mol/L SP and 3 x 10 (-5) mol/L spantide added to the culture medium) groups. MTT method or flow cytometry assay was used for the determination of the proliferative activities or apoptotic rate of fibroblasts obtained from KSF, HSF and NDF with SP or Spantide. And then the fibroblasts in SP group were subdivided into 1 x 10( -9) -1 x 10 (-5) mol/L groups to examine the time-or dose-effect of SP to fibroblasts from different sources.
In control group, different types of fibroblasts exhibited similar proliferative activities and apoptotic rates. But there was significant difference in these indices between control and SP group (the proliferative activity of KSF, HSF, NDF was 0. 656+/-0. 071, 0. 525 +/-0. 064, 0. 404+/-0. 063, respectively; and the apoptotic rate of KSF, HSF, NDF was [( 1.5+/-0.3) % , (4.0+/-0.5) % , (5.5+/-0.7) % , respectively],( P < 0. 05). SP had stronger effect on KSF than it did to HSF, as well as it had stronger effect on HSF than it did to NDF. In SP + spantide group, the effect of SP on KSF was partially inhibited, while it was completely inhibited in cultures of HSF and NDF. KSF was more sensitive to SP and the effect was longer when compared with HSF.
SP may play an important role in the process of pathological scar formation due to its diverse effects on fibroblasts from different sources.
进一步探讨P物质对体外培养的病理性瘢痕成纤维细胞增殖和凋亡的影响。
取12例烧伤患者瘢痕疙瘩（KSF）、增生性瘢痕（HSF）及正常皮肤真皮（NDF）的成纤维细胞进行体外培养，分为对照组、SP组（培养基中加入1×10⁻⁶mol/L P物质）和SP + spantide组（培养基中加入1×10⁻⁶mol/L P物质和3×10⁻⁵mol/L spantide）。采用MTT法或流式细胞术检测P物质或spantide作用下KSF、HSF和NDF成纤维细胞的增殖活性或凋亡率。然后将SP组的成纤维细胞再分为1×10⁻⁹ - 1×10⁻⁵mol/L组，检测P物质对不同来源成纤维细胞的时间或剂量效应。
对照组中，不同类型的成纤维细胞增殖活性和凋亡率相似。但对照组与SP组之间这些指标存在显著差异（KSF、HSF、NDF的增殖活性分别为0.656±0.071、0.525±0.064、0.404±0.063；KSF、HSF、NDF的凋亡率分别为[(1.5±0.3)%、(4.0±0.5)%、(5.5±0.7)%]，P < 0.05）。P物质对KSF的作用强于对HSF的作用，对HSF的作用强于对NDF的作用。在SP + spantide组中，P物质对KSF的作用部分被抑制，而在HSF和NDF培养物中完全被抑制。与HSF相比，KSF对P物质更敏感，且作用时间更长。
P物质对不同来源的成纤维细胞有不同作用，可能在病理性瘢痕形成过程中起重要作用。
