急性髓系白血病M2及t(8;19)(q22;q13)

[Acute myeloid leukemia M2and t (8; 19) (q22; q13)].

作者信息

Li P, Guo Y, Xie X, Wang Y, Lu D, Xue Y

机构信息

Jiangsu Institute of Hematology, First Affiliated Hospital of Suzhou Medical College, Suzhou 215006, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2000 Feb;21(2):84-6.

PMID:11876965

Abstract

OBJECTIVE

Report for the first time of two cases of acute myeloid leukemia (AML) M(2) with t (8; 19) (q22; q13).

METHODS

Chromosome specimens were prepared by short-term culture of bone marrow cells and karyotype analyses were carried out using R-and G-banding techniques. Immunophenotyping of the blast cells was analyzed by flow cytometry with a panel of monoclonal antibodies. AML1/ETO fusion gene was tested by "nested" reverse transcriptase-polymerase chain reaction (RT-PCR).

RESULTS

Karyotype analyses showed 46, XX, t (8; 19) (q22; q13) [28]/46, XX [2] in case 1 and t (8; 19) (q22; q13), del (9) (q12q22) [23]/46, XY [2] in case 2. In case 2, the blast cells expressed CD(13) (38.8%), CD(33) (31.8%), CD(34) (80.9%), and CD(19) (63.9%) and RT-PCR assay revealed no AML1/ETO fusion gene transcript.

CONCLUSION

t (8; 19) (q22; q13) is a variant form of t (8; 21) (q22; q22). Its molecular entity remains to be elucidate.

摘要

目的

首次报告两例伴t（8；19）（q22；q13）的急性髓系白血病（AML）M2病例。

方法

通过骨髓细胞短期培养制备染色体标本，并采用R显带和G显带技术进行核型分析。采用一组单克隆抗体通过流式细胞术分析原始细胞的免疫表型。通过“巢式”逆转录聚合酶链反应（RT-PCR）检测AML1/ETO融合基因。

结果

核型分析显示，病例1为46,XX,t（8；19）（q22；q13）[28]/46,XX[2]，病例2为t（8；19）（q22；q13），del（9）（q12q22）[23]/46,XY[2]。在病例2中，原始细胞表达CD13（38.8%）、CD33（31.8%）、CD34（80.9%）和CD19（63.9%），RT-PCR检测未发现AML1/ETO融合基因转录本。