[热处理的人慢性粒细胞白血病细胞对自体T细胞杀伤的体外诱导]

[In vitro induction of autologous T cell killing by heat treated human chronic myelogenous leukemia cells].

作者信息

He X, You S, Bian S, Liao X, Li M, Ma S, Ge W, Qian L

机构信息

Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC., Tianjin 300020, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2000 Jun;21(6):287-90.

PMID:11876993

Abstract

OBJECTIVE

To investigate the potential of autologous T cell killing of heat treated chronic myelogenous leukemia (CML) cells (autologous tumor killing, ATK).

METHODS

(51)Cr release assay was used to measure the ATK activity of autologous T cells against CML cells treated with 42 degrees C for 30 minutes (heat) or 37 degrees C for 30 minutes (non-heat). The phenotypes of T cells and heat shock protein 70 (HSP70) expression of CML cells were measured by flow cytometry (FCM). T cells from the CML patients were stimulated and expanded by autologous mixed lymphocyte/tumor cell cultures (MLTC).

RESULTS

ATK activity of autologous T cells to the non-heated and heated CML cells were found in 4 (19.05%) and 10 (47.62%) of the 21 cases, respectively. The ATK activity of interleukin-2 (IL-2) stimulated autologous T cells against heated CML cells was markedly higher than that of unstimulated autologous T cells against non-heated CML cells (P < 0.001). FCM analysis showed that no HSP70 was expressed on the CML cell membranes whether heated or non-heated, but intracellular HSP70 expressions were (83.42 +/- 5.65)% and (78.34 +/- 6.32)% pre- and post-heated, respectively. The phenotypes of T cells stimulated and expanded in MLTC were TCRgammadelta - CD(3)(+), mostly CD(8)(+), with some activation markers (CD(25) and HLA-DR) expression. The ATK activities of these T cells against the heated and non-heated CML cells and K562 cells were (51.25 +/- 4.26)%, (36.52 +/- 3.83)% and (2.92 +/- 1.19)%, respectively.

CONCLUSIONS

ATK activity of autologous T cells against CML cells could be induced or enhanced by heat treatment of the CML cells particularly of T cells stimulated with IL-2. This ATK activity was not associated with gammadelta T cells or HSP70 expression of CML cells.

摘要

目的

研究自体T细胞对热处理慢性髓性白血病（CML）细胞的杀伤潜力（自体肿瘤杀伤，ATK）。

方法

采用（51）Cr释放试验检测自体T细胞对经42℃处理30分钟（热）或37℃处理30分钟（非热）的CML细胞的ATK活性。通过流式细胞术（FCM）检测T细胞表型及CML细胞热休克蛋白70（HSP70）表达。慢性髓性白血病患者的T细胞通过自体混合淋巴细胞/肿瘤细胞培养（MLTC）进行刺激和扩增。

结果

21例患者中，分别有4例（19.05%）和10例（47.62%）的自体T细胞对未加热和加热后的CML细胞具有ATK活性。白细胞介素-2（IL-2）刺激的自体T细胞对加热后CML细胞的ATK活性明显高于未刺激的自体T细胞对未加热CML细胞的ATK活性（P<0.001）。FCM分析显示，无论加热与否，CML细胞膜上均未表达HSP70，但加热前后细胞内HSP70表达分别为（83.42±5.65）%和（78.34±6.32）%。在MLTC中刺激和扩增的T细胞表型为TCRγδ-CD(3)(+)，大多为CD(8)(+)，伴有一些活化标志物（CD(25)和HLA-DR）表达。这些T细胞对加热和未加热的CML细胞及K562细胞的ATK活性分别为（51.25±4.26）%、（36.52±3.83）%和（2.92±1.19）%。