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椰子(Cocos nucifera L.)杂交种及其亲本的油中脂肪酸和三酰甘油组成的变异性。

Variability in fatty acid and triacylglycerol composition of the oil of coconut (Cocos nucifera L.) hybrids and their parentals.

作者信息

Laureles Lucita R, Rodriguez Felicito M, Reaño Consorcia E, Santos Gerardo A, Laurena Antonio C, Mendoza Evelyn Mae Tecson

机构信息

Institute of Plant Breeding, College of Agriculture, University of the Philippines Los Baños, College 4031, Laguna, Philippines.

出版信息

J Agric Food Chem. 2002 Mar 13;50(6):1581-6. doi: 10.1021/jf010832w.

Abstract

The fatty acid profiles and triacylglycerol (TAG) compositions of oils from the solid endosperm of different Philippine coconut hybrids and their parentals were determined by using gas chromatography (GC) and high-performance liquid chromatography (HPLC). In general, varietal differences in fatty acid composition were observed. Lauric acid (C12) content was significantly higher in the hybrids PCA 15-8 (50.45%) and PCA 15-9 (50.26%) by about 3.16% points as compared to other hybrids, and higher in Tacunan Green Dwarf (50.50%) among the parentals. Among the fatty acids, lauric acid exhibited the least variation. In general, none of the hybrids had higher fatty acid content than their parentals. The HPLC chromatogram of triacylglycerols (TAG) showed 8 major peaks which differ in carbon number (CN) by two: identified as TAG CN 30, 32, 34, 36, 38, 40, 42, and 44. TAGs CN 30 (4.08%) and CN 34 (19.20%) were found to be significantly higher in PCA 15-9 than in the other hybrids. CN 36 was highest (21.94-23.66%) in all hybrids and parentals. The TAG CNs varied significantly among hybrids and parents, i.e., in CN 30, 32, and 34, which are high in medium chain triacylglycerols (MCTs), and in CN 30 (for parentals only), 40, 42, and 44 (the latter two for parentals only), and none in CN 36. MCTs calculated for two hybrids and their parents ranged from 13.81% to 20.55%.

摘要

采用气相色谱法(GC)和高效液相色谱法(HPLC)测定了不同菲律宾椰子杂交种及其亲本固体胚乳油的脂肪酸谱和三酰甘油(TAG)组成。总体而言,观察到脂肪酸组成存在品种差异。杂交种PCA 15 - 8(50.45%)和PCA 15 - 9(50.26%)中的月桂酸(C12)含量比其他杂交种显著高约3.16个百分点,在亲本中,塔库南绿矮种(50.50%)的月桂酸含量更高。在脂肪酸中,月桂酸的变化最小。总体而言,没有一个杂交种的脂肪酸含量高于其亲本。三酰甘油(TAG)的HPLC色谱图显示有8个主要峰,其碳数(CN)相差2:分别鉴定为TAG CN 30、32、34、36、38、40、42和44。发现PCA 15 - 9中TAG CN 30(4.08%)和CN 34(19.20%)显著高于其他杂交种。CN 36在所有杂交种和亲本中最高(21.94 - 23.66%)。TAG CN在杂交种和亲本之间差异显著,即在中链三酰甘油(MCT)含量高的CN 30、32和34中,以及在CN 30(仅亲本)、40、42和44(后两者仅亲本)中存在差异,而CN 36中没有差异。两个杂交种及其亲本的MCT计算值在13.81%至20.55%之间。

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