Balderan Ali, Farrokhifar Yasamin, Hosseini Mahmoud, Khordad Elnaz, Askarian Saeedeh, Kakhki Samaneh, Beheshti Farimah
Student Research Committee, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Avicenna J Phytomed. 2023 Nov-Dec;13(6):665-674. doi: 10.22038/AJP.2023.22724.
The current study aimed to investigate whether L. oil (CO) is effective on menopause-related memory dysfunction in ovariectomized (OVX) rats.
Fifty healthy female Wistar rats were randomly selected and classified into five groups as control, OVX rats, and three OVX groups of rats which received three different doses (100, 200, and 400 mg/kg/day) of CO for five consecutive weeks by gavage. To assess the effect of CO, neurobehavioral tests such as Morris water maze (MWM) and Passive avoidance (PA) were done and then the animals were sacrificed to remove cortical and hippocampal tissues for biochemical analysis.
In both behavioral tests including MWM and PA, treatment with CO particularly two higher doses of 200, and 400 mg/kg demonstrated significant improvement in comparison with OVX group. Furthermore, antioxidant biomarkers such as total thiol content, catalase and superoxide dismutase (SOD) activities were significantly higher in the OVX-CO groups versus the OVX group. On the contrary, malondialdehyde (MDA) concentration as an oxidative stress biomarker was remarkably lower in the OVX-CO200 and 400 mg groups than the OVX group.
The present study demonstrated the significant improvement of CO on learning and memory impairment induced by ovariectomy. Although the exact mechanism needs further investigation, it might have occurred due to the anti-oxidative effect of CO.
本研究旨在探讨月见草油（CO）对去卵巢（OVX）大鼠绝经相关记忆功能障碍是否有效。
随机选取50只健康雌性Wistar大鼠，分为五组，即对照组、OVX大鼠组，以及三个OVX大鼠组，后三组大鼠连续五周通过灌胃给予三种不同剂量（100、200和400mg/kg/天）的CO。为评估CO的作用，进行了Morris水迷宫（MWM）和被动回避（PA）等神经行为测试，然后处死动物以取出皮质和海马组织进行生化分析。
在包括MWM和PA的两项行为测试中，与OVX组相比，CO处理尤其是200和400mg/kg这两种较高剂量显示出显著改善。此外，OVX-CO组的抗氧化生物标志物如总硫醇含量、过氧化氢酶和超氧化物歧化酶（SOD）活性明显高于OVX组。相反，作为氧化应激生物标志物的丙二醛（MDA）浓度在OVX-CO200和400mg组中明显低于OVX组。
本研究表明CO对去卵巢诱导的学习和记忆损伤有显著改善作用。尽管确切机制需要进一步研究，但可能是由于CO的抗氧化作用所致。
