未变性的无绿原酸向日葵（向日葵属）蛋白质的分离与表征
Isolation and characterization of undenatured chlorogenic acid free sunflower (Helianthus annuus) proteins.
作者信息
González-Pérez Sergio, Merck Karin B, Vereijken Johan M, van Koningsveld Gerrit A, Gruppen Harry, Voragen Alphons G J
机构信息
ATO, P.O. Box 17, 6700AA Wageningen, The Netherlands.
出版信息
J Agric Food Chem. 2002 Mar 13;50(6):1713-9. doi: 10.1021/jf011245d.
A method for obtaining sunflower protein (SFP) isolate, nondenatured and free of chlorogenic acid (CGA), has been developed. During the isolating procedure, the extent of CGA removal and protein denaturation was monitored. The defatted flour contained 2.5% CGA as the main phenolic compound. Phenolic compounds were removed by aqueous methanol (80%) extraction, before protein extraction at alkaline pH and diafiltration. Differential scanning calorimetry and solubility tests indicated that no denaturation of the proteins had occurred. The resulting protein products were biochemically characterized, and the presence of protein-CGA complexes was investigated. SFPs of the studied variety were found to be composed of two main protein fractions: 2S albumins and 11S globulins. In contrast to what has been previously reported, CGA was found to elute as free CGA, not covalently associated to any protein fraction.
已开发出一种获得未变性且不含绿原酸（CGA）的向日葵分离蛋白（SFP）的方法。在分离过程中，监测了CGA去除程度和蛋白质变性情况。脱脂面粉中含有2.5%的CGA作为主要酚类化合物。在碱性pH值下进行蛋白质提取和渗滤之前，通过80%的甲醇水溶液萃取去除酚类化合物。差示扫描量热法和溶解度测试表明蛋白质未发生变性。对所得蛋白质产品进行了生化表征，并研究了蛋白质-CGA复合物的存在情况。研究品种的SFP由两个主要蛋白质组分组成：2S白蛋白和11S球蛋白。与先前报道的情况相反，发现CGA以游离CGA的形式洗脱，而非与任何蛋白质组分共价结合。
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