Chung Hwan-Suck, Jeong Hyun-Ja, Kim Jung-Soo, Jeong Seung-Il, Kim Kyung-Soo, Kim Kang-San, Kang Byung-Ki, Ahn Jong-Woong, Baek Seung-Hwa, Kim Hyung-Min
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, Chonbuk 570-749, South Korea.
Clin Chim Acta. 2002 Apr;318(1-2):113-20. doi: 10.1016/s0009-8981(01)00808-7.
Euonymus alatus (EA) has been used for tumor therapy. However, it is still unclear how this herb prevents the diseases in experimental models. Nitric oxide (NO) as a potent macrophage-derived effector molecule against a variety of tumors has received increasing attention.
Using mouse peritoneal macrophages, we have examined the mechanism by which EA regulates NO production.
When EA was used in combination with recombinant interferon-gamma (rIFN-gamma), there was a marked cooperative induction of NO production. However, EA had no effect on NO production by itself. The increased production of NO from rIFN-gamma plus EA-stimulated cells was almost completely inhibited by pre-treatment with pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor kappa B (NF-kappaB). Furthermore, treatment of peritoneal macrophages with rIFN-gamma plus EA caused a significant increase in tumor necrosis factor-alpha (TNF-alpha) production. PDTC also decreased the effects of EA on TNF-alpha production significantly.
EA increases the production of NO and TNF-alpha by rIFN-gamma-primed macrophages and suggest that NF-kappaB plays a critical role in mediating these effects of EA.
卫矛已被用于肿瘤治疗。然而，在实验模型中这种草药如何预防疾病仍不清楚。一氧化氮（NO）作为一种由巨噬细胞产生的针对多种肿瘤的有效效应分子，受到了越来越多的关注。
我们使用小鼠腹腔巨噬细胞研究了卫矛调节NO产生的机制。
当卫矛与重组干扰素-γ（rIFN-γ）联合使用时，NO产生有明显的协同诱导作用。然而，卫矛本身对NO产生没有影响。用核因子κB（NF-κB）抑制剂吡咯烷二硫代氨基甲酸盐（PDTC）预处理，几乎完全抑制了rIFN-γ加卫矛刺激的细胞中NO产生的增加。此外，用rIFN-γ加卫矛处理腹腔巨噬细胞导致肿瘤坏死因子-α（TNF-α）产生显著增加。PDTC也显著降低了卫矛对TNF-α产生的影响。
卫矛增加了rIFN-γ预处理的巨噬细胞中NO和TNF-α的产生，并表明NF-κB在介导卫矛的这些作用中起关键作用。
Zotero 插件