白屈菜水提取物通过激活小鼠腹腔巨噬细胞中的核因子-κB增强一氧化氮和肿瘤坏死因子-α的产生。

Water extract isolated from Chelidonium majus enhances nitric oxide and tumour necrosis factor-alpha production via nuclear factor-kappaB activation in mouse peritoneal macrophages.

作者信息

Chung Hwan-Suck, An Hyo-Jin, Jeong Hyun-Ja, Won Jin-Hee, Hong Seung-Heon, Kim Hyung-Min

机构信息

Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoigi-Dong, Dongdaemun-gu, Seoul 130-701, South Korea.

出版信息

J Pharm Pharmacol. 2004 Jan;56(1):129-34. doi: 10.1211/0022357022467.

DOI:10.1211/0022357022467

PMID:14980010

Abstract

Chelidonium majus is used to treat several inflammatory diseases and tumours. We have examined the effect of C. majus on nitric oxide (NO) production using mouse peritoneal macrophages. When C. majus was used in combination with recombinant interferon-gamma (rIFN-gamma, 10 U mL(-1)), there was a marked cooperative induction of NO production. Treatment of rIFN-gamma plus C. majus (1 mgmL(-1)) in macrophages caused a significant increase in tumour necrosis factor-alpha (TNF-alpha) production. The increased production of NO and TNF-alpha from rIFN-gamma plus C. majus-stimulated cells was almost completely inhibited by nuclear factor-kappaB (NF-kappaB) inhibitor, pyrrolidine dithiocarbamate (100 microM). These findings demonstrated that C. majus increased the production of NO and TNF-alpha by rIFN-gamma-primed macrophages and suggested that NF-kappaB played a critical role in mediating the effects of C. majus.

摘要

白屈菜用于治疗多种炎症性疾病和肿瘤。我们使用小鼠腹腔巨噬细胞研究了白屈菜对一氧化氮（NO）生成的影响。当白屈菜与重组干扰素-γ（rIFN-γ，10 U mL⁻¹）联合使用时，对NO生成有显著的协同诱导作用。在巨噬细胞中用rIFN-γ加白屈菜（1 mgmL⁻¹）处理导致肿瘤坏死因子-α（TNF-α）生成显著增加。rIFN-γ加白屈菜刺激的细胞中NO和TNF-α生成的增加几乎被核因子-κB（NF-κB）抑制剂吡咯烷二硫代氨基甲酸盐（100 microM）完全抑制。这些发现表明，白屈菜增加了rIFN-γ预处理的巨噬细胞中NO和TNF-α的生成，并提示NF-κB在介导白屈菜的作用中起关键作用。