采用聚苯乙烯-二乙烯基苯反相高效液相色谱法检测和定量分析牛乳清样品中的乳铁蛋白。

Detection and quantitation of lactoferrin in bovine whey samples by reversed-phase high-performance liquid chromatography on polystyrene-divinylbenzene.

作者信息

Palman Kate P, Elgar David F

机构信息

Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand.

出版信息

J Chromatogr A. 2002 Feb 22;947(2):307-11. doi: 10.1016/s0021-9673(01)01563-1.

DOI:10.1016/s0021-9673(01)01563-1

PMID:11883664

Abstract

An existing RP-HPLC method for the measurement of the major bovine whey proteins in bovine whey samples and powders has been extended to include the analysis of the minor bovine whey protein lactoferrin. Lactoferrin could be detected and quantitated at levels down to 0.2 microg and linear calibration was observed between 0.2 and 30 microg. Reliable quantitation of lactoferrin in whey samples could be achieved provided the bovine serum albumin to lactoferrin ratio did not exceed 10:1. Quantitative data obtained by the RP-HPLC method compared favourably with data obtained by Mono S analytical chromatography.

摘要

一种用于测定牛乳清样品和乳清粉中主要牛乳清蛋白的现有反相高效液相色谱（RP-HPLC）方法已得到扩展，以包括对次要牛乳清蛋白乳铁蛋白的分析。乳铁蛋白能够在低至0.2微克的水平被检测和定量，并且在0.2至30微克之间观察到线性校准。只要牛血清白蛋白与乳铁蛋白的比例不超过10:1，就能实现对乳清样品中乳铁蛋白的可靠定量。通过RP-HPLC方法获得的定量数据与通过Mono S分析色谱法获得的数据相比具有优势。