Oyama Tetsunari, Sano Takaaki, Hikino Toshiaki, Xue Qi, Iijima Kotaro, Nakajima Takashi, Koerner Frederick
Second Department of Pathology, Gunma University School of Medicine, Showa-machi 3-39-22, Maebashi, Gunma 371-8511, Japan.
Virchows Arch. 2002 Mar;440(3):267-73. doi: 10.1007/s004280100501. Epub 2001 Aug 24.
We studied the pattern of calcification and the expression of osteopontin protein and mRNA by the histiocytes and noninvasive carcinoma cells of 20 breast cancers and the histiocytes and atypical ductal cells of sixteen cases of atypical cystic lobules. Ten breast cancers showed low-grade cribriform carcinoma in situ containing secretory material; the remaining ten cancers displayed high-grade carcinoma in situ with central necrosis characteristic of comedo type carcinoma. Hematoxylin-eosin and Kossa staining revealed calcium hydroxyapatite calcifications in 80% of cribriform type carcinomas, 50% of comedo type carcinomas, and 56% of atypical cystic lobules. Immunohistochemical staining demonstrated osteopontin protein in intraluminal secretory material, necrotic debris, or stroma, and in histiocytes in all the Kossa-positive carcinomas and atypical cystic lobules. In situ hybridization revealed osteopontin mRNA mainly in the histiocytes and especially in those near the calcifications. In two cases, rare carcinoma cells contained osteopontin protein and mRNA. The close relation between hydroxyapatite crystals and osteopontin-producing histiocytes suggests that osteopontin plays a role in the biomineralization that occurs in certain noninvasive breast cancers and atypical cystic lobules. The differences in the morphology of the calcifications and the intraductal contents suggest that the mechanism leading to the osteopontin production might vary depending on the underlying lesion.
我们研究了20例乳腺癌的组织细胞和非侵袭性癌细胞以及16例非典型性囊性小叶的组织细胞和非典型性导管细胞中钙化的模式、骨桥蛋白的蛋白质及信使核糖核酸的表达情况。10例乳腺癌显示为含有分泌物质的低级别筛状原位癌；其余10例癌表现为高级别原位癌，具有粉刺样癌特征性的中央坏死。苏木精-伊红染色和柯萨染色显示，80%的筛状型癌、50%的粉刺样癌和56%的非典型性囊性小叶中有羟基磷灰石钙化。免疫组织化学染色显示，在所有柯萨染色阳性的癌和非典型性囊性小叶中，腔内分泌物质、坏死碎片或基质以及组织细胞中有骨桥蛋白。原位杂交显示，骨桥蛋白信使核糖核酸主要存在于组织细胞中，尤其是在钙化灶附近的组织细胞中。在2例中，罕见的癌细胞含有骨桥蛋白和信使核糖核酸。羟基磷灰石晶体与产生骨桥蛋白的组织细胞之间的密切关系表明，骨桥蛋白在某些非侵袭性乳腺癌和非典型性囊性小叶中发生的生物矿化过程中发挥作用。钙化形态和导管内内容物的差异表明，导致骨桥蛋白产生的机制可能因潜在病变而异。
