Kerskes C H M, Lusthof K J, Zweipfenning P G M, Franke J P
The Netherlands Forensic Institute, Department Toxicology, Rijswijk.
J Anal Toxicol. 2002 Jan-Feb;26(1):29-34. doi: 10.1093/jat/26.1.29.
Quaternary nitrogen muscle relaxants pancuronium, rocuronium, vecuronium, gallamine, suxamethonium, mivacurium, and atracurium and its metabolites were extracted from whole blood and other biological fluids and tissues by using a solid-phase extraction procedure. The extracts were examined by using high-performance liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS). The drugs were separated on a ODS column in a gradient of ammonium acetate buffer (pH 5.0) and acetonitrile. Full-scan mass spectra of the compounds showed molecular ions, and MS-MS spectra showed fragments typical of the particular compounds. LC-ESI-MS allowed an unequivocal differentiation of all muscle relaxants involved. The method was applied in a case of rocuronium and suxamethonium administration in a Caesarian section and in a case of intoxication by pancuronium injection. In both cases, the administered drugs could be detected and identified in the supplied samples.
采用固相萃取法从全血及其他生物体液和组织中提取季铵类肌肉松弛剂泮库溴铵、罗库溴铵、维库溴铵、加拉明、琥珀胆碱、米库氯铵、阿曲库铵及其代谢产物。提取物采用高效液相色谱 - 电喷雾电离质谱法（LC - ESI - MS）进行检测。药物在乙酸铵缓冲液（pH 5.0）和乙腈梯度的ODS柱上分离。化合物的全扫描质谱显示分子离子，MS - MS谱显示特定化合物的典型碎片。LC - ESI - MS能够明确区分所有涉及的肌肉松弛剂。该方法应用于剖宫产术中罗库溴铵和琥珀胆碱给药的病例以及泮库溴铵注射中毒的病例。在这两种情况下，所供应样品中均可检测和鉴定出所施用的药物。
