Freitas D S, Maia I G, Arruda P, Vega J
Departamento de Fisiologia Vegetal, Universidade Estadual de Campinas, SP, Brazil.
Arch Virol. 2002;147(2):411-7. doi: 10.1007/s705-002-8329-1.
The 3' terminal genomic region of a potyvirus causing mosaic disease in several Crotalaria species has been cloned and sequenced. Comparisons of the nucleotide and deduced amino acid (aa) sequences of the cloned cDNA with those from other potyviruses show that the Crotalaria-infecting virus (designated Crotalaria mosaic virus; CrMV) is closely related to Cowpea aphid-borne mosaic virus (CABMV). Maximum identity (95.4%) at the coat protein (CP) aa level was observed between CrMV and a Brazilian strain of CABMV. Phylogenetic analyses derived from the sequence alignments of the CP and 3' untranslated region confirmed the identification of CrMV as a strain of CABMV and the name CABMV-Cr is suggested.
已克隆并测序了一种在几种猪屎豆属植物中引起花叶病的马铃薯Y病毒属病毒的3'末端基因组区域。将克隆的cDNA的核苷酸序列和推导的氨基酸(aa)序列与其他马铃薯Y病毒属病毒的序列进行比较,结果表明,感染猪屎豆属植物的病毒(命名为猪屎豆花叶病毒;CrMV)与豇豆蚜传花叶病毒(CABMV)密切相关。在CrMV与巴西的一个CABMV株系之间,在衣壳蛋白(CP)氨基酸水平上观察到最大同一性(95.4%)。基于CP和3'非翻译区序列比对的系统发育分析证实CrMV为CABMV的一个株系,并建议将其命名为CABMV-Cr。