一种感染向日葵的新型马铃薯Y病毒的分子特征分析
Molecular characterization of a new potyvirus infecting sunflower.
作者信息
Dujovny G, Sasaya T, Koganesawa H, Usugi T, Shohara K, Lenardon S L
机构信息
Instituto de Fitopatología y Fisiología Vegetal-Instituto Nacional de Tecnología Agropecuaria, Córdoba, Argentina.
出版信息
Arch Virol. 2000;145(11):2249-58. doi: 10.1007/s007050070018.
We have sequenced 1873 nucleotides from the 3'-end region of a sunflower potyvirus genome including the 3'-NIb protein coding region (813 nucleotides), the entire coat protein coding region (807 nucleotides) and 3'-NCR (253 nucleotides), excluding the poly (A) tail. Amino acids identity of the whole CP between the sunflower virus and Potyvirus members ranged from 49.5% (SCMV) to 81.5% (PVY-NsNr), and the core ranged from 55% (TVMV) to 87% (PVY-NsNr; PepMoV). The 3'-NCR nucleotides showed 38.7% homology to PeSMV and 61% to PepMoV-C. The sequence of 3' end region and analysis of phylogenetic relationships suggest this sunflower virus could belong to PVY subgroup and the name of "sunflower chlorotic mottle virus" (SuCMoV) is proposed. This is the first report on the partial nucleotide sequence of a potyvirus infecting sunflower.
我们对一种向日葵马铃薯Y病毒基因组3'-末端区域的1873个核苷酸进行了测序，包括3'-NIb蛋白编码区（813个核苷酸）、整个外壳蛋白编码区（807个核苷酸）和3'-非编码区（253个核苷酸），不包括聚（A）尾。向日葵病毒与马铃薯Y病毒属成员之间整个外壳蛋白的氨基酸同一性范围为49.5%（甘蔗花叶病毒）至81.5%（马铃薯Y病毒NsNr株系），核心区域的同一性范围为55%（烟草脉斑驳病毒）至87%（马铃薯Y病毒NsNr株系；辣椒脉斑驳病毒）。3'-非编码区核苷酸与花生斑驳病毒的同源性为38.7%，与辣椒脉斑驳病毒C株系的同源性为61%。3'末端区域的序列及系统发育关系分析表明，这种向日葵病毒可能属于马铃薯Y病毒亚组，并提出了“向日葵褪绿斑驳病毒”（SuCMoV）这一名称。这是关于一种感染向日葵的马铃薯Y病毒部分核苷酸序列的首次报道。
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