Lee J C, Oh J Y, Kim K S, Jeong Y W, Park J C, Cho J W
Department of Microbiology, College of Medicine, Seonam University, Namwon, Chunpook, Korea.
APMIS. 2001 Oct;109(10):679-84. doi: 10.1034/j.1600-0463.2001.d01-132.x.
Epithelial cell death induced by Acinetobacter baumannii infection was investigated using in vitro assays. Eight hours after live A. baumannii infection, HeLa cells exhibited detachment from the dish, rounding morphologies, high proportions of trypan blue-positive cells and extensive DNA breakdown with faint apoptotic banding, which is indicative of cells undergoing apoptosis. The enzymatic activity of caspase-3 was increased in cells as early as 2 h after infection. In addition, apoptosis of HeLa cells was induced by treatment with bacterial culture filtrates but not with formalin-killed bacteria. These results indicate that A. baumannii infection triggers apoptosis in HeLa cells through caspase-3 activation.
利用体外试验研究了鲍曼不动杆菌感染诱导的上皮细胞死亡。活的鲍曼不动杆菌感染8小时后，HeLa细胞出现从培养皿上脱落、形态变圆、高比例的台盼蓝阳性细胞以及广泛的DNA断裂和微弱的凋亡条带，这表明细胞正在经历凋亡。感染后仅2小时，细胞中caspase-3的酶活性就升高了。此外，用细菌培养滤液处理可诱导HeLa细胞凋亡，而用福尔马林灭活的细菌处理则不能。这些结果表明，鲍曼不动杆菌感染通过激活caspase-3触发HeLa细胞凋亡。
