Tiwari Vishvanath, Tiwari Monalisa, Solanki Vandana
Department of Biochemistry, Central University of Rajasthan, Ajmer, India.
Front Immunol. 2017 Aug 16;8:973. doi: 10.3389/fimmu.2017.00973. eCollection 2017.
, an opportunistic ESKAPE pathogen, causes respiratory and urinary tract infections. Its prevalence increases gradually in the clinical setup. Pathogenicity of is significantly influenced by its ability to infect and survive in human pulmonary cells. Therefore, it is important to study the infection of in human pulmonary host cell (A-549), monitoring surface interacting and internalized bacteria. It was found that during infection of , about 40% bacteria adhered to A-549, whereas 20% got internalized inside pulmonary cell and induces threefold increase in the reactive oxygen species production. We have synthesized polyvinylpyrrolidone (PVP)-capped AgNPs using chemical methods and tested its efficacy against carbapenem-resistant strain of . PVP-capped silver nanoparticles (PVP-AgNPs) (30 µM) have shown antibacterial activity against carbapenem-resistant strain of and this concentration does not have any cytotoxic effect on the human pulmonary cell line (IC is 130 µM). Similarly, PVP-AgNPs treatment decreases 80% viability of intracellular bacteria, decreases adherence of to A-549 (40 to 2.2%), and decreases intracellular concentration (20 to 1.3%) of . This concludes that PVP-AgNPs can be developed as a substitute for carbapenem to control the infection caused by carbapenem-resistant .
作为一种机会性ESKAPE病原体，可引起呼吸道和泌尿道感染。其在临床环境中的患病率逐渐上升。的致病性受到其在人肺细胞中感染和存活能力的显著影响。因此，研究在人肺宿主细胞（A-549）中的感染情况，监测表面相互作用和内化的细菌很重要。研究发现，在感染期间，约40%的细菌粘附在A-549上，而20%的细菌内化到肺细胞内，并导致活性氧产生增加三倍。我们使用化学方法合成了聚乙烯吡咯烷酮（PVP）包覆的银纳米颗粒（AgNPs），并测试了其对耐碳青霉烯类菌株的疗效。PVP包覆的银纳米颗粒（PVP-AgNPs）（30µM）对耐碳青霉烯类菌株显示出抗菌活性，且该浓度对人肺细胞系没有任何细胞毒性作用（半数抑制浓度为130µM）。同样，PVP-AgNPs处理可降低细胞内细菌80%的活力，降低对A-549的粘附率（从40%降至2.2%），并降低的细胞内浓度（从20%降至1.3%）。由此得出结论，PVP-AgNPs可作为碳青霉烯类药物的替代品来控制耐碳青霉烯类引起的感染。
