Yoon Seung-Heon, Robyt John F
Laboratory of Carbohydrate Chemistry and Enzymology, Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
Carbohydr Res. 2002 Mar 15;337(6):509-16. doi: 10.1016/s0008-6215(02)00018-6.
New kinds of acarbose analogues were synthesized by the reaction of acarbose with cyclomaltohexaose and cyclomaltodextrin glucanyltransferase (CGTase). Three major CGTase coupling products were separated and purified by Bio-Gel P2 gel-permeation chromatography. Digestion of the three products by beta-amylase and glucoamylase showed that they were composed of maltohexaose (G6), maltododecaose (G12), and maltooctadecaose (G18), respectively, attached to the nonreducing-end of acarbose. 13C NMR of the glucoamylase product (D-glucopyranosyl-acarbose) showed that the D-glucose moiety was attached alpha- to the C-4-OH group of the nonreducing-end cyclohexene ring of acarbose, indicating that the maltodextrins were attached alpha-(1-->4) to the nonreducing-end cyclohexene of acarbose.
通过阿卡波糖与环麦芽六糖和环糊精葡糖基转移酶（CGTase）反应合成了新型阿卡波糖类似物。通过Bio-Gel P2凝胶渗透色谱法分离并纯化了三种主要的CGTase偶联产物。用β-淀粉酶和葡糖淀粉酶对这三种产物进行消化表明，它们分别由连接在阿卡波糖非还原端的麦芽六糖（G6）、麦芽十二糖（G12）和麦芽十八糖（G18）组成。葡糖淀粉酶产物（D-吡喃葡萄糖基-阿卡波糖）的13C NMR表明，D-葡萄糖部分以α-方式连接到阿卡波糖非还原端环己烯环的C-4-OH基团上，这表明麦芽糊精以α-(1→4)方式连接到阿卡波糖的非还原端环己烯上。
