通过阿卡波糖与环麦芽六糖和环糊精葡糖基转移酶反应，将麦芽糊精添加到阿卡波糖的非还原端。

Addition of maltodextrins to the nonreducing-end of acarbose by reaction of acarbose with cyclomaltohexaose and cyclomaltodextrin glucanyltransferase.

作者信息

Yoon Seung-Heon, Robyt John F

机构信息

Laboratory of Carbohydrate Chemistry and Enzymology, Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA 50011, USA.

出版信息

Carbohydr Res. 2002 Mar 15;337(6):509-16. doi: 10.1016/s0008-6215(02)00018-6.

DOI:10.1016/s0008-6215(02)00018-6

PMID:11890888

Abstract

New kinds of acarbose analogues were synthesized by the reaction of acarbose with cyclomaltohexaose and cyclomaltodextrin glucanyltransferase (CGTase). Three major CGTase coupling products were separated and purified by Bio-Gel P2 gel-permeation chromatography. Digestion of the three products by beta-amylase and glucoamylase showed that they were composed of maltohexaose (G6), maltododecaose (G12), and maltooctadecaose (G18), respectively, attached to the nonreducing-end of acarbose. 13C NMR of the glucoamylase product (D-glucopyranosyl-acarbose) showed that the D-glucose moiety was attached alpha- to the C-4-OH group of the nonreducing-end cyclohexene ring of acarbose, indicating that the maltodextrins were attached alpha-(1-->4) to the nonreducing-end cyclohexene of acarbose.

摘要

通过阿卡波糖与环麦芽六糖和环糊精葡糖基转移酶（CGTase）反应合成了新型阿卡波糖类似物。通过Bio-Gel P2凝胶渗透色谱法分离并纯化了三种主要的CGTase偶联产物。用β-淀粉酶和葡糖淀粉酶对这三种产物进行消化表明，它们分别由连接在阿卡波糖非还原端的麦芽六糖（G6）、麦芽十二糖（G12）和麦芽十八糖（G18）组成。葡糖淀粉酶产物（D-吡喃葡萄糖基-阿卡波糖）的13C NMR表明，D-葡萄糖部分以α-方式连接到阿卡波糖非还原端环己烯环的C-4-OH基团上，这表明麦芽糊精以α-(1→4)方式连接到阿卡波糖的非还原端环己烯上。