Yang S M, Jing S, Doi T, Kaneko T, Yamashita T
Department of Otolaryngology, Kansai Medical University, Osaka, Japan.
ORL J Otorhinolaryngol Relat Spec. 2002 Jan-Feb;64(1):1-5. doi: 10.1159/000049078.
Cochlear inner hair cells (IHCs) and outer hair cells (OHCs) show distinctive morphological features that are usually sufficient to distinguish these two species in vitro. However, OHCs may sometimes resemble IHCs when they are mechanically distorted or begin swelling at their basal end. As a result, accurately discriminating the cells based on morphology was thought to be problematic. An objective method that allows us to clearly and unambiguously distinguish these two cell types is therefore of continued interest. We describe a protocol in which solitary IHCs were harvested from guinea pig cochlea using a manual microsurgical dissection.
耳蜗内毛细胞（IHC）和外毛细胞（OHC）呈现出独特的形态特征，这些特征通常足以在体外区分这两种细胞类型。然而，当外毛细胞受到机械扭曲或其基部开始肿胀时，它们有时可能类似于内毛细胞。因此，基于形态准确区分这些细胞被认为存在问题。因此，一种能够让我们清晰明确地区分这两种细胞类型的客观方法一直备受关注。我们描述了一种通过手动显微手术解剖从豚鼠耳蜗中获取单个内毛细胞的方法。
