Lainchbury John G, Lisy Ondrej, Burnett John C, Meyer Donna M, Redfield Margaret M
Cardiorenal Research Laboratory, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA.
Am J Physiol Regul Integr Comp Physiol. 2002 Apr;282(4):R993-8. doi: 10.1152/ajpregu.00388.2001.
Dendroaspis natriuretic peptide (DNP) is a recently discovered peptide with structural similarity to known natriuretic peptides. DNP has been shown to possess potent renal actions. Our objectives were to define the acute hemodynamic actions of DNP in normal anesthetized dogs and the acute effects of DNP on left ventricular (LV) function in conscious chronically instrumented dogs. In anesthetized dogs, DNP, but not placebo, decreased mean arterial pressure (141 +/- 6 to 109 +/- 7 mmHg, P < 0.05) and pulmonary capillary wedge pressure (5.8 +/- 0.3 to 3.4 +/- 0.2 mmHg, P < 0.05). Cardiac output decreased and systemic vascular resistance increased with DNP and placebo. DNP-like immunoreactivity and guanosine 3',5'-cyclic monophosphate concentration increased without changes in other natriuretic peptides. In conscious dogs, DNP decreased LV end-systolic pressure (120 +/- 7 to 102 +/- 6 mmHg, P < 0.05) and volume (32 +/- 6 to 28 +/- 6 ml, P < 0.05) and LV end-diastolic volume (38 +/- 5 to 31 +/- 4 ml, P < 0.05) but not arterial elastance. LV end-systolic elastance increased (6.1 +/- 0.7 to 7.4 +/- 0.6 mmHg/ml, P < 0.05), and Tau decreased (31 +/- 2 to 27 +/- 1 ms, P < 0.05). The effects on hemodynamics, LV function, and second messenger generation suggest synthetic DNP may have a role as a cardiac unloading and lusitropic peptide.
树眼镜蛇利钠肽（DNP）是一种最近发现的肽，其结构与已知的利钠肽相似。已证明DNP具有强大的肾脏作用。我们的目标是确定DNP在正常麻醉犬中的急性血流动力学作用，以及DNP对清醒慢性植入仪器犬左心室（LV）功能的急性影响。在麻醉犬中，DNP而非安慰剂降低了平均动脉压（从141±6 mmHg降至109±7 mmHg，P<0.05）和肺毛细血管楔压（从5.8±0.3 mmHg降至3.4±0.2 mmHg，P<0.05）。使用DNP和安慰剂时，心输出量降低，全身血管阻力增加。DNP样免疫反应性和鸟苷3',5'-环磷酸浓度增加，而其他利钠肽无变化。在清醒犬中，DNP降低了左心室收缩末期压力（从120±7 mmHg降至102±6 mmHg，P<0.05）、容积（从32±6 ml降至28±6 ml，P<0.05）和左心室舒张末期容积（从38±5 ml降至31±4 ml，P<0.05），但未降低动脉弹性。左心室收缩末期弹性增加（从6.1±0.7 mmHg/ml增至7.4±0.6 mmHg/ml，P<0.05），等容舒张时间常数降低（从31±2 ms降至27±1 ms，P<0.05）。对血流动力学、左心室功能和第二信使生成的影响表明，合成DNP可能具有心脏减负和促舒张肽的作用。
