Sankovic Snezana, Sofronic Ljiljana
ORL. Clinic, Clinical Hospital Centre Zvezdara, Belgrade, Institute for Application of Nuclear Energy-INEP, Zemun.
Acta Otorhinolaryngol Belg. 2002;56(1):61-4.
Carcinoembryonic antigen (CEA) has an important role as a tumor marker and also as a cell adhesion molecule. The expression and the role of CEA in chronic inflammation of middle ear mucosa has not been studied previously. Immunohistochemical analysis with monoclonal antibodies against human CEA was performed in order to detect CEA in formalin fixed and paraffin embedded biopsy specimens of middle ear mucosa in chronic otitis media. In this study 118 specimens of middle ear mucosa from different regions of the middle ear cleft were analysed. All specimens were taken during surgery of otitis media with and without cholesteatoma. The results showed that CEA expression was present in mucosal samples in chronic otitis media with cholesteatoma, predominantly in squamous epithelium, as well as in the subepithelial layer and among the connective tissue, especially on collagen fibres of fibroproliferative stroma. CEA was seen on endothelial cells of blood vessels. Occasionally it was present on the surface of columnar epithelium of the middle ear mucosa in chronic otitis media without cholesteatoma. The expression patterns of CEA in chronic inflammation of middle ear mucosa suggest potential functional activities similar to adhesion molecules and signal regulatory proteins which will be the subject of further study.
癌胚抗原（CEA）作为一种肿瘤标志物以及细胞黏附分子发挥着重要作用。此前尚未对CEA在中耳黏膜慢性炎症中的表达及作用进行研究。为了在慢性中耳炎福尔马林固定石蜡包埋的中耳黏膜活检标本中检测CEA，采用了抗人CEA单克隆抗体进行免疫组织化学分析。本研究分析了来自中耳裂不同区域的118例中耳黏膜标本。所有标本均在伴有或不伴有胆脂瘤的中耳炎手术期间采集。结果显示，在伴有胆脂瘤的慢性中耳炎黏膜样本中存在CEA表达，主要见于鳞状上皮，以及上皮下层和结缔组织中，尤其在纤维增生性基质的胶原纤维上。在血管内皮细胞上可见CEA。在不伴有胆脂瘤的慢性中耳炎中，偶尔在中耳黏膜柱状上皮表面也有CEA存在。中耳黏膜慢性炎症中CEA的表达模式提示其可能具有与黏附分子和信号调节蛋白类似的功能活性，这将是进一步研究的主题。
