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一种荧光法测定人脑脊液中5-羟吲哚乙酸的简化方法(作者译)

[A simplified method for the fluorometric determination of 5-hydroxy-indoleacetic acid in the human liquor cerebrospinalis (author's transl)].

作者信息

Geissbühler F

出版信息

Z Klin Chem Klin Biochem. 1975 Jul;13(7):283-4.

PMID:1189529
Abstract

The fluorometric analysis of 5-hydroxy-indoleacetic acid by condensation with o-phthaldialdehyde has been modified by substituting a HCl-H2SO4 mixture at room temperature for hot concentrated HCl. The sensitivity of the method was increased by a factor of 2.7.

摘要

通过用室温下的盐酸 - 硫酸混合物替代热的浓盐酸,改进了邻苯二甲醛缩合荧光法对5 - 羟基吲哚乙酸的分析。该方法的灵敏度提高了2.7倍。

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